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| Status |
Public on Oct 01, 2009 |
| Title |
CREB ChIP-chip and HaloCHIP-chip experiments |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
Regulation of gene expression is essential for normal development and cellular growth. Transcriptional events are tightly controlled both spatially and temporally by specific DNA-protein interactions. In this study we finely map the genome-wide targets of the CREB protein across all known and predicted human promoters, and characterize the functional consequences of a subset of these binding events using high-throughput reporter assays. To measure CREB binding, we used HaloCHIP, an antibody-free alternative to the ChIP method that utilizes the HaloTag fusion protein, and also high-throughput promoter-luciferase reporter assays, which provide rapid and quantitative screening of promoters for transcriptional activation or repression in living cells.
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| Overall design |
CREB ChIP-chip two biologcal replicates. HaloCHIP-chip three biological replicates with and without Forskolin
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| Contributor(s) |
Hartzell DD, Trinklein ND, Mendez J, Murphy N, Aldred SF, Wood K, Urh M |
| Citation(s) |
19860899 |
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| Submission date |
Sep 30, 2009 |
| Last update date |
Nov 04, 2013 |
| Contact name |
Danette Hartzell |
| E-mail(s) |
danette.hartzell@promega.com
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| Organization name |
Promega
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| Street address |
2800 Woods Hollow Road
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| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53711 |
| Country |
USA |
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| Platforms (1) |
| GPL9325 |
NimbleGen Human 385K custom array [2007-05-01_SwitchGear_hg17_tiling] |
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| Samples (9)
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| GSM458034 |
ChIP-chip CREB + Forskolin biological rep 1, technical rep 1 |
| GSM458035 |
ChIP-chip CREB + Forskolin biological rep 2, technical rep 1 |
| GSM458036 |
HaloCHIP CREB + Forskolin biological rep 1, technical rep 1 |
| GSM458037 |
HaloCHIP CREB + Forskolin biological rep 2, technical rep 1 |
| GSM458038 |
HaloCHIP CREB + Forskolin biological rep 3, technical rep 1 |
| GSM458039 |
HaloCHIP CREB + Forskolin biological rep 1, technical rep 2 |
| GSM458040 |
HaloCHIP CREB (no Forskolin) biological rep 1 |
| GSM458041 |
HaloCHIP CREB (no Forskolin) biological rep 2 |
| GSM458042 |
HaloCHIP CREB (no Forskolin) biological rep 3 |
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| Relations |
| BioProject |
PRJNA118157 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18347_HaloCHIP_alldata.txt.gz |
796.5 Kb |
(ftp)(http) |
TXT |
| GSE18347_RAW.tar |
90.6 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE18347_chip_chip_alldata.txt.gz |
440.1 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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