|
| Status |
Public on Feb 03, 2022 |
| Title |
WIN site inhibition disrupts a subset of WDR5 function |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
|
| Summary |
This study demonstrates the impact of WIN site inhibitors versus WDR5 degradation on H3K4me and transcriptional processes in human Burkitt's lymphoma cells. We use RNA-seq to measure global transcript levels, ChIP-seq to map genomic H3K4me3, and PRO-seq to map genomic polymerase density and primary transcripts. Our data show that WIN site inhibition disables only a specific subset of WDR5 activity, and that H3K4me changes induced by WDR5 depletion do not explain accompanying transcriptional responses.
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| |
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| Overall design |
Degrade WDR5 rapidly with Auxin (IAA) in AIDW Ramos cells and inhibit WDR5 WIN-site with C16 inhibitor.
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| |
|
| Contributor(s) |
Tansey WP, Siladi AJ, Thomas LR, Wang J, Liu Q |
| Citation(s) |
35115608 |
| |
| Submission date |
Sep 09, 2021 |
| Last update date |
May 05, 2022 |
| Contact name |
Jing Wang |
| Organization name |
Vanderbilt University Medical Center
|
| Street address |
2220 Pierce Avenue
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (38)
|
|
| Relations |
| BioProject |
PRJNA761980 |
| SRA |
SRP336342 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE183781_IAA_Me3_N1_peaks.narrowPeak.gz |
345.7 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183781_IAA_Me3_N2_peaks.narrowPeak.gz |
339.9 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183781_IAA_Me3_N3_peaks.narrowPeak.gz |
330.1 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183781_NT_Me3_N1_peaks.narrowPeak.gz |
414.4 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183781_NT_Me3_N2_peaks.narrowPeak.gz |
418.9 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183781_NT_Me3_N3_peaks.narrowPeak.gz |
409.8 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE183781_PROseq_count_pp_gb.txt.gz |
1.9 Mb |
(ftp)(http) |
TXT |
| GSE183781_RNAseq_proteincoding_count.txt.gz |
900.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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