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| Status |
Public on Nov 09, 2021 |
| Title |
Transcriptional profiling to elucidate processes regulated by 2',3'-cyclic nucleotide phosphodiesterase (CNPase) in Salmonella Typhimurium 14028s (Salmonella) II. |
| Organism |
Salmonella enterica subsp. enterica serovar Typhimurium |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The impact of 2',3'-cyclic nucleotide phosphodiesterase (CNPase) on global gene expression in Salmonella was investigated by heterologous expression of mammalian CNPase in a strain containing a deletion of the 'rna' gene. 'rna' encodes an endoribonuclease capable of hydrolyzing RNA into 2',3'-cyclic nucleotide monophosphates (2',3'-cNMPs) such that deletion of 'rna' drastically reduces 2',3'-cNMPs presence to undetectable levels. Expression of CNPase in this strain allowed any effects of CNPase unrelated to 2',3'-cNMPs to be observed.
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| Overall design |
Global transcript levels in a 'rna' deletion strain of Salmonella expressing CNPase were compared to levels in control cells with the same deletion expressing a catalytically inactive variant of CNPase in order to identify cellular processes modulated by CNPase that are unrelated to 2',3'-cyclic nucleotide monophosphates.
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| Contributor(s) |
Fontaine BM, Kurasz JE, Duggal Y, Chauhan SS, Marotta N, Karls AC, Weinert EE |
| Citation(s) |
34662237 |
| |
| Submission date |
Sep 15, 2021 |
| Last update date |
Feb 08, 2022 |
| Contact name |
Emily Weinert |
| E-mail(s) |
eew5225@psu.edu
|
| Organization name |
Pennsylvania State University
|
| Department |
Biochemistry and Molecular Biology
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| Street address |
306 Althouse Laboratory
|
| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
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| Platforms (1) |
| GPL30637 |
NextSeq 2000 (Salmonella enterica subsp. enterica serovar Typhimurium) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA763526 |
| SRA |
SRP337233 |
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