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| Status |
Public on Sep 01, 2023 |
| Title |
DNA methylation landscape and signature of CD4+ lymphocytes of lamina propria in Crohn's patients by reduced representation and bisulfite sequencing [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A genome-wide DNA methylation study was conducted using base resolution bisulfite sequencing for purified intestinal CD4+ cells (resected or biopsied mucosa of a terminal ileum) from 21 patients with Crohn’s disease and 12 age/sex matched controls. The sequencing data was analyzed for differentially methylated CpGs (DMCs) and differentially methylated regions (DMRs). Integration was performed with RNA-sequencing data for the same set of samples to verify the functional impact of DNA methylation changes.
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| |
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| Overall design |
Crohn’s disease; CD4+ lymphocytes; DNA methylation; reduced representation bisulfite sequencing; differential methylation; Th17 phenotype; ATAC-sq
Case/control study
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| |
|
| Contributor(s) |
Sun Z, Neto MB, Xiong Y, Baheti S, Friton J, Nair A, Sagstetter M, Ye Z, Faubion W |
| Citation(s) |
37280154 |
| |
| Submission date |
Sep 16, 2021 |
| Last update date |
Dec 01, 2023 |
| Contact name |
ZHIFU SUN |
| Organization name |
Mayo Clinic
|
| Department |
Quantitative Health Sciences
|
| Street address |
200 1st St SW
|
| City |
ROCHESTER |
| State/province |
Minnesota |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (33)
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| This SubSeries is part of SuperSeries: |
| GSE184314 |
DNA methylation landscape and signature of CD4+ lymphocytes of lamina propria in Crohn's patients by reduced representation and bisulfite sequencing |
|
| Relations |
| BioProject |
PRJNA763930 |
| SRA |
SRP337477 |
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