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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 30, 2021 |
| Title |
10X genomics single cell sequencing of sheep testis |
| Organism |
Ovis aries |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Sheep testes undergo a dramatic rate of development with structural changes during sexual maturity, including the proliferation and maturation of somatic niche cells and the initiation of spermatogenesis. To explore this complex process, 12,843 testicular cells from three sexual maturity (3 month-old) rams were sequenced using 10x Genomic platform single-cell sequencing (scRNA-seq).Nine testicular somatic cell types and five male germ cell types were observed.The study revealed significant changes in germline stem cells during sexual maturation. Candidate factors and pathways for the regulation of germ and somatic cell development were identified that represent the scientific basis for the development of a livestock stem cell breeding program.
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| Overall design |
mRNA profile of one Hu sheep testis sample was generated by single cell sequencing
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| Contributor(s) |
Yang B, Yue Y, Wu Y |
| Citation(s) |
35723319 |
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| Submission date |
Sep 17, 2021 |
| Last update date |
Jun 24, 2022 |
| Contact name |
Yao jing Yue |
| E-mail(s) |
yueyaojing@126.com
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| Phone |
86-0931-211-5273
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| Organization name |
Chinese Academy of Agricultural Sciences
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| Department |
Lanzhou Institute of Husbandry and Pharmaceutical Sciences,
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| Lab |
Sheep Breeding Engineering Technology Center
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| Street address |
NO.335 Jiangouyan, Qilihe District Lanzhou 730050, Gansu, P. R. C
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| City |
Lanzhou |
| ZIP/Postal code |
730050 |
| Country |
China |
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| Platforms (1) |
| GPL27721 |
Illumina NovaSeq 6000 (Ovis aries) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA764185 |
| SRA |
SRP337619 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE184343_RAW.tar |
61.5 Mb |
(http)(custom) |
TAR (of MTX, TSV, XLSX) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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