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| Status |
Public on Sep 16, 2024 |
| Title |
Conditional deletion of HIF-2α in mouse nucleus pulposus reduces fibrosis and provides mild and transient protection from age-dependent structural changes in intervertebral disc |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Hypoxia inducible factors (HIFs) are critical to the development and homeostasis of the intervertebral disc. While HIF-2α, one of the main HIF-α isoforms is expressed in nucleus pulposus (NP) cells, its functions remain unknown.
We used microarrays to explore the transcriptomics of differentially expressed genes between control and HIF-2α cKO NP cells in 6M and 14M mice.
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| Overall design |
NP tissue were dissected from discs from control (n=4) and HIF-2α cKO mice (n=4). Mice were aged until they became 6-months-old or 14-months-old. RNA extraction was prefomed and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Johnston SN, Risbud MV |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 20, 2021 |
| Last update date |
Sep 16, 2024 |
| Contact name |
Shira N Johnston |
| E-mail(s) |
snj007@students.jefferson.edu
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| Organization name |
Thomas Jefferson University
|
| Street address |
1025 Walnut St., Suite 511 College Bldg.
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (16)
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| GSM5588603 |
6M CT, NP 4 |
| GSM5588604 |
6M cKO, NP 1 |
| GSM5588605 |
6M cKO, NP 2 |
| GSM5588606 |
6M cKO, NP 3 |
| GSM5588607 |
6M cKO, NP 4 |
| GSM5588608 |
14M CT, NP 1 |
| GSM5588609 |
14M CT, NP 2 |
| GSM5588610 |
14M CT, NP 3 |
| GSM5588611 |
14M CT, NP 4 |
| GSM5588612 |
14M cKO, NP 1 |
| GSM5588613 |
14M cKO, NP 2 |
| GSM5588614 |
14M cKO, NP 3 |
| GSM5588615 |
14M cKO, NP 4 |
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| Relations |
| BioProject |
PRJNA764605 |
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