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| Status |
Public on Nov 16, 2021 |
| Title |
Analysis of the antiviral activity of TIP against SARS-CoV2 in Mesocricetus auratus |
| Organism |
Mesocricetus auratus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Syrian golden hamsters were treated with TIP or control RNA and infected with SARS-CoV2, at 5 days post infection, RNA was extracted and RNAseq was performed, uninfected hamsters treated with TIP or Control RNA were used as controls.
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| Overall design |
Samples 1-10: 8-week old syrian golden hamsters were infected through intranasal installation of 10^6 total PFU per animal of SARS-CoV-2 (USA-WA1/2020) 100 ml of DMEM, as described (Rogers et al., 2020). 6 hours pre-infection and 18 hours post infection animals were given an intranasal dose of 100 ml of LNP solution containing 60 mg of either TIP RNA (hamsters n=5; samples 1-5) or control RNA (hamsters n=5; samples 6-10). For uninfected samples; 8-week old syrian golden hamsters were intranasally treated with TIP (hamsters n=3; samples 11-13) or control RNA LNPs (hamsters n=3; samples 14-16). At day-5 post-infection, animals were sacrificed and lungs were harvested for gene expression analysis.
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| Contributor(s) |
Chaturvedi S, Chen X, Beutler N |
| Citation(s) |
34838159 |
| |
| Submission date |
Sep 20, 2021 |
| Last update date |
Feb 15, 2022 |
| Contact name |
Xinyue Chen |
| Organization name |
Gladstone Institutes
|
| Street address |
1650 Owens St
|
| City |
San Francisco |
| State/province |
California |
| ZIP/Postal code |
94158 |
| Country |
USA |
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| Platforms (1) |
| GPL24490 |
Illumina NextSeq 500 (Mesocricetus auratus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA764687 |
| SRA |
SRP337876 |
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