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| Status |
Public on May 15, 2010 |
| Title |
Expression analysis of Clostridium acetobutylicum ATCC 824 grown on different carbohydrates |
| Organism |
Clostridium acetobutylicum ATCC 824 |
| Experiment type |
Expression profiling by array
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| Summary |
Investigation of whole genome gene expression levels in Clostridium acetobutylicum strain 824 grown individually on starch, galactose, lactose, mannose, fructose, glucose, arabinose, xylose, cellobiose, maltose, sucrose
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| Overall design |
A 33 array study using total RNA recovered from three separate wild-type cultures of Clostridium acetobutylicum 824 grown on each of the following sugars: starch, galactose, lactose, mannose, fructose, glucose, arabinose, xylose, cellobiose, maltose, sucrose. RNA extraction, cDNA production, and labeling were performed independently on three separate cultures for each carbohydrate. Each independent sample was hybridized to one array equating to one technical replicate for each biological sample and three biological replicates for each condition tested. Each array contains 2 replicates of 9 probes approximately 60 bp in length for each of the 3,842 open reading frames annotated in the C. acetobutylicum 824 genome.
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| Contributor(s) |
Sund CJ, Servinsky MD, Kiel JT, Dupuy NF |
| Citation(s) |
20656779 |
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| Submission date |
Oct 07, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Christian Sund |
| Organization name |
US Army Research Laboratory
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| Street address |
2800 Powder Mill Road
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| City |
Adelphi |
| State/province |
MD |
| ZIP/Postal code |
20783 |
| Country |
USA |
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| Platforms (1) |
| GPL9387 |
NimbleGen Clostridium acetobutylicum ATCC 824 4-plex 3.8K expression array |
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| Samples (33)
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| GSM459919 |
C. acetobutylicum Glucose Replicate 3 |
| GSM459920 |
C. acetobutylicum Glucose Replicate 2 |
| GSM459921 |
C. acetobutylicum Glucose Replicate 1 |
| GSM459922 |
C. acetobutylicum Cellobiose Replicate 3 |
| GSM459923 |
C. acetobutylicum Cellobiose Replicate 2 |
| GSM459939 |
C. acetobutylicum Cellobiose Replicate 1 |
| GSM459940 |
C. acetobutylicum Galactose Replicate 3 |
| GSM459941 |
C. acetobutylicum Galactose Replicate 2 |
| GSM459942 |
C. acetobutylicum Galactose Replicate 1 |
| GSM459943 |
C. acetobutylicum Starch Replicate 3 |
| GSM459944 |
C. acetobutylicum Starch Replicate 2 |
| GSM459945 |
C. acetobutylicum Starch Replicate 1 |
| GSM459946 |
C. acetobutylicum Sucrose Replicate 3 |
| GSM459947 |
C. acetobutylicum Sucrose Replicate 1 |
| GSM459948 |
C. acetobutylicum Sucrose Replicate 2 |
| GSM459949 |
C. acetobutylicum Arabinose replicate 3 |
| GSM459950 |
C. acetobutylicum Arabinose Replicate 2 |
| GSM459969 |
C. acetobutylicum Arabinose Replicate 1 |
| GSM459970 |
C. acetobutylicum Mannose Replicate 3 |
| GSM459999 |
C. acetobutylicum Mannose Replicate 2 |
| GSM460000 |
C. acetobutylicum Mannose Replicate 1 |
| GSM460001 |
C. acetobutylicum Maltose Replicate 3 |
| GSM460002 |
C. acetobutylicum Maltose Replicate 2 |
| GSM460003 |
C. acetobutylicum Maltose Replicate 1 |
| GSM460004 |
C. acetobutylicum Fructose Replicate 1 |
| GSM460005 |
C. acetobutylicum Fructose Replicate 2 |
| GSM460006 |
C. acetobutylicum Fructose Replicate 3 |
| GSM460007 |
C. acetobutylicum Xylose Replicate 3 |
| GSM460008 |
C. acetobutylicum Xylose Replicate 2 |
| GSM460009 |
C. acetobutylicum Xylose Replicate 1 |
| GSM460010 |
C. acetobutylicum Lactose Replicate 1 |
| GSM460011 |
C. acetobutylicum Lactose Replicate 2 |
| GSM460012 |
C. acetobutylicum Lactose Replicate 3 |
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| Relations |
| BioProject |
PRJNA118201 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18471_RAW.tar |
41.1 Mb |
(http)(custom) |
TAR (of PAIR) |
| Processed data included within Sample table |
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