NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE184719 Query DataSets for GSE184719
Status Public on Feb 23, 2022
Title Human-induced CD49a+ NK cells promote fetal growth
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary CD49a+ natural killer (NK) cells are critical in promoting fetal development and maintaining immune tolerance at the maternal-fetal interface in early pregnancy. However, their tissue residency in human tissue hinder thorough studies and clinical application. How to induce functional human CD49a+ NK cells that could benefit pregnancy outcomes is still unknown. Here, we have established three no feeder cell induction systems to induce human CD49a+ NK cells from umbilical cord blood hematopoietic stem cells (HSCs), bone marrow HSCs or peripheral blood NK cells, respectively. These induced NK cells (iNKs) from three cell induction systems show high expression of CD49a, CD9, CD39, CD151, low expression of CD16, and no obvious cytotoxic capability, phenotypically and functionally similar with decidual NK cells. Furthermore, these iNKs also have high expression of growth-promoting factors and proangiogenic factors. Importantly, these iNKs have shown their capabilities to promote fetal growth and improve uterine artery blood flow in a murine pregnancy model in vivo. This research reveals properties of human-induced CD49a+ NK cells in promoting fetal growth from three cell induction systems, which may improve the feasibility of applying these iNKs to the patients having adverse pregnancy outcomes.
 
Overall design The mRNA profiles of decidual NK cells, peripheral blood NK cells, induced CD49a+ NK cells from umbilical cord blood hematopoietic stem cells (HSCs), bone marrow HSCs or peripheral blood NK cells. Samples were all from healthy people.
 
Contributor(s) Du X
Citation(s) 35185911
Submission date Sep 24, 2021
Last update date Feb 23, 2022
Contact name Xianghui Du
E-mail(s) duxh@mail.ustc.edu.cn
Phone +8617855122373
Organization name Division of Life Sciences and Medicine
Department Institute of Immunology
Lab Wei Lab
Street address 443 Huangshan Road
City Hefei
State/province Anhui
ZIP/Postal code 230027
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (20)
GSM5596121 dNK1
GSM5596122 dNK2
GSM5596123 dNK3
Relations
BioProject PRJNA765937
SRA SRP338559

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE184719_gene_counts.txt.gz 667.6 Kb (ftp)(http) TXT
GSE184719_gene_fpkm.txt.gz 705.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.