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Status |
Public on Oct 04, 2021 |
Title |
15-Lipoxygenase-2 Deficiency Induces a Dysfunction in Macrophages that can be Restored by Salidroside |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
15-Lipoxygenase-2 (15-LOX-2) is thought to regulate inflammation and immunological function; however, its mechanisms of action are still unclear. Furthermore, it has been reported that salidroside has anti-inflammatory properties, but its role in macrophage function is not yet understood. In this study, we aimed to determine how 15-LOX-2 expression levels affect the function of macrophages and the effect of salidroside on 15-LOX-2-deficient macrophages. We used multiple functional genetic strategies to determine 15-LOX-2 function in macrophages. 15-LOX-2 deficiency promoted phagocytosis and the proliferation of macrophages and impaired their apoptosis. Mechanistically, the expression levels of cyclophilin B (CypB) were upregulated in 15-LOX-2-deficient Ana-1 macrophages, whereas those of caspase-3 were downregulated. Furthermore, RNA-seq analysis showed that inflammation, complement, and TNF-α signaling pathways were all activated in 15-LOX-2-deficient Ana-1 macrophages. Treatment of 15-LOX-2-deficient macrophages with salidroside, a natural product derived from Rhodiola species, effectively reversed the effects of 15-LOX-2 deficiency on caspase-3 and CypB levels, as well as on apoptosis and proliferation. In conclusion, our study shows a newly identified link between 15-LOX-2 deficiency and salidroside in regulating macrophage survival, proliferation, and function. Salidroside may be a promising therapeutic strategy for treating inflammation-related diseases resulting from 15-LOX-2 deficiency.
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Overall design |
RNA-seq data of 15-LOX-2 deficient Ana-1 macrophage
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Contributor(s) |
Zhou C, Xu Z, Li T, Yong X, Wen H, Huang R |
Citation missing |
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Submission date |
Sep 27, 2021 |
Last update date |
Oct 04, 2021 |
Contact name |
zhengmin xu |
E-mail(s) |
xuzhengmin@NSMC.edu.cn
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Organization name |
North Sichuan Medical College
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Department |
Department of Pharmacy
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Street address |
Fujiang
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City |
Nanchong |
ZIP/Postal code |
637000 |
Country |
China |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (10)
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Relations |
BioProject |
PRJNA766638 |
SRA |
SRP338898 |
Supplementary file |
Size |
Download |
File type/resource |
GSE184840_RNAseq_TPM.xlsx |
2.9 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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