NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE184840 Query DataSets for GSE184840
Status Public on Oct 04, 2021
Title 15-Lipoxygenase-2 Deficiency Induces a Dysfunction in Macrophages that can be Restored by Salidroside
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary 15-Lipoxygenase-2 (15-LOX-2) is thought to regulate inflammation and immunological function; however, its mechanisms of action are still unclear. Furthermore, it has been reported that salidroside has anti-inflammatory properties, but its role in macrophage function is not yet understood. In this study, we aimed to determine how 15-LOX-2 expression levels affect the function of macrophages and the effect of salidroside on 15-LOX-2-deficient macrophages. We used multiple functional genetic strategies to determine 15-LOX-2 function in macrophages. 15-LOX-2 deficiency promoted phagocytosis and the proliferation of macrophages and impaired their apoptosis. Mechanistically, the expression levels of cyclophilin B (CypB) were upregulated in 15-LOX-2-deficient Ana-1 macrophages, whereas those of caspase-3 were downregulated. Furthermore, RNA-seq analysis showed that inflammation, complement, and TNF-α signaling pathways were all activated in 15-LOX-2-deficient Ana-1 macrophages. Treatment of 15-LOX-2-deficient macrophages with salidroside, a natural product derived from Rhodiola species, effectively reversed the effects of 15-LOX-2 deficiency on caspase-3 and CypB levels, as well as on apoptosis and proliferation. In conclusion, our study shows a newly identified link between 15-LOX-2 deficiency and salidroside in regulating macrophage survival, proliferation, and function. Salidroside may be a promising therapeutic strategy for treating inflammation-related diseases resulting from 15-LOX-2 deficiency.
 
Overall design RNA-seq data of 15-LOX-2 deficient Ana-1 macrophage
 
Contributor(s) Zhou C, Xu Z, Li T, Yong X, Wen H, Huang R
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Sep 27, 2021
Last update date Oct 04, 2021
Contact name zhengmin xu
E-mail(s) xuzhengmin@NSMC.edu.cn
Organization name North Sichuan Medical College
Department Department of Pharmacy
Street address Fujiang
City Nanchong
ZIP/Postal code 637000
Country China
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (10)
GSM5598060 shRen-1
GSM5598061 shRen-2
GSM5598062 shRen-3
Relations
BioProject PRJNA766638
SRA SRP338898

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE184840_RNAseq_TPM.xlsx 2.9 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.