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| Status |
Public on Jan 30, 2022 |
| Title |
Tig1 regulates proximo-distal identity during salamander limb regeneration |
| Organism |
Ambystoma mexicanum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Salamander limb regeneration is an accurate process which gives rise exclusively to the missing structures, irrespective of the amputation level. This suggests that cells in the stump have an awareness of their spatial location, a property termed ‘positional identity’. Little is known about how positional identity is encoded, in salamanders or other biological systems. Through single-cell RNAseq analysis, we identified Tig1/RARRES1 as a potential determinant of proximal identity. Tig1 encodes a conserved cell surface molecule, is regulated by retinoic acid and exhibits a graded expression along the proximo-distal axis of the limb. Its overexpression leads to regeneration defects in the distal elements and elicits proximal displacement of blastema cells, while its neutralisation blocks proximo-distal cell surface interactions. Critically, Tig1 reprogrammes distal cells to a proximal identity, upregulating Prod1 and inhibiting HoxA13 and distal transcriptional networks. Thus, Tig1 is a central cell surface determinant of proximal identity in the salamander limb.
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| Overall design |
Transcriptomic changes elicited by Tig1-transfected vs. Non-transfected axolotl distal forelimb blastema cells (Hoxa13+).
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| Contributor(s) |
Oliveira CR, Knapp D, Gerber T, Elewa A, Gonzalez-Malagon SG, Gates PB, Walters HE, Petzold A, Arce H, Cordoba RC, Subramanian E, Chara O, Tanaka EM, Simon A, Yun MH |
| Citation(s) |
35241664 |
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| Submission date |
Sep 28, 2021 |
| Last update date |
May 01, 2022 |
| Contact name |
Maximina Yun |
| E-mail(s) |
maximina.yun@tu-dresden.de
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| Organization name |
TUD
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| Department |
CRTD
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| Lab |
YUN
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| Street address |
Fetscherstrasse 105
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| City |
Dresden |
| State/province |
Saxony |
| ZIP/Postal code |
01307 |
| Country |
Germany |
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| Platforms (1) |
| GPL24679 |
Illumina NextSeq 500 (Ambystoma mexicanum) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA767085 |
| SRA |
SRP339156 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE184948_bfx1392.AmexG_v6_DD.eAmexG_v6_DD.txt.gz |
2.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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