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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 25, 2022 |
| Title |
Induction of mouse totipotent stem cells by a defined chemical cocktail [RRBS] |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We demonstrate induction and long-term maintenance of totipotent stem cells (TotiSCs) from mouse pluripotent stem cells (PSCs) by a combination of three small molecules, TTNPB, 1-Azakenpaullone, and WS6. These cells, which we designated as ciTotiSCs (chemically induced totipotent stem cells), resembled mouse totipotent 2C-embryo stage cells at both transcriptome and epigenome level.
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| Overall design |
RRBS-seq of ciTotiSC with two replicates.
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| Contributor(s) |
Hu Y, Yang Y, Tan P, Zhang Y, Yu J, Zhang X, Jia Z, Wang D, Li Y, Ma T, Liu K, Ding S |
| Citation(s) |
35728625 |
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| Submission date |
Sep 29, 2021 |
| Last update date |
Jun 27, 2022 |
| Contact name |
Pengcheng Tan |
| E-mail(s) |
tpchengbjhd@126.com
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| Organization name |
Tsinghua University
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| Street address |
30 Shuangqing Rd
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| City |
Haidian |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE185005 |
Induction of mouse totipotent stem cells by a defined chemical cocktail |
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| Relations |
| BioProject |
PRJNA767358 |
| SRA |
SRP339302 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE185004_RAW.tar |
21.5 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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