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| Status |
Public on Feb 04, 2022 |
| Title |
Human Pluripotent Stem Cell-derived Islets Ameliorate Diabetes in Nonhuman Primates [human_bulk] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Human pluripotent stem cell-derived islets (hPSC-islets) are a promising cell resource for diabetes treatment. Here, we demonstrate that transplantation of human pluripotent stem cell-derived islets into diabetic nonhuman primates effectively restored endogenous insulin secretion and improved glycemic control. Single-cell RNA sequencing analysis of S6D2 clusters confirmed the existence of the three major pancreatic endocrine cell populations (β cells, α-like cells and δ-like cells) and their proportions, which altogether accounted for 80%. Importantly, hierarchical clustering of S6D2 hCiPSC-islets, 10 wpt kidney grafts and primary human islets showed that the hCiPSC differentiated pancreatic endocrine cells shared similar global gene expression profiles to their native counterparts in primary human islets. Single-cell RNA sequencing analysis on PBMCs revealed the potential immune response of recipient macaque to hCiPSC-islets.
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| Overall design |
Bulk RNA sequencing analysis on hADFs, hCiPSCs, hiPSCs and hESCs to compare the global gene expression profiles.
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| Contributor(s) |
Du Y, Wang Y, Liang Z, Wang S, Sun D, Wang X, Yi LS, Lu S |
| Citation(s) |
35115708 |
| Submission date |
Sep 29, 2021 |
| Last update date |
Feb 04, 2022 |
| Contact name |
Yaqi Wang |
| E-mail(s) |
yaqi_wang@pku.edu.cn
|
| Organization name |
Peking University
|
| Street address |
No.5 Yiheyuan Road Haidian District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE185038 |
Human Pluripotent Stem Cell-derived Islets Ameliorate Diabetes in Nonhuman Primates |
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| Relations |
| BioProject |
PRJNA767419 |
| SRA |
SRP339353 |
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