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Series GSE185196 Query DataSets for GSE185196
Status Public on Oct 01, 2022
Title How thermal challenges change gene regulation in the songbird brain and gonad: implications for sexual selection in our changing world 
Organism Taeniopygia guttata
Experiment type Expression profiling by high throughput sequencing
Summary In a rapidly warming world, exposure to high temperatures may impact fitness, but the gene regulatory mechanisms that link sublethal heat to sexually selected traits are not well understood, particularly in endothermic animals. Our experiment used zebra finches (Taeniopygia guttata), songbirds that experience extreme temperature fluctuations in their native Australia. We exposed captive males to an acute thermal challenge (43°C) compared with thermoneutral (35°C) and lower (27°C) temperatures. We found significantly more heat dissipation behaviors at 43°C and heat retention behaviors at 27°C, temperatures previously shown to reduce song production and fertility. Next, we characterized gene expression in tissues important for mating effort – the posterior telencephalon, for its role in song production, and the testis, for its role in fertility and hormone production. Differential expression of hundreds of genes in the testes, but few in the brain, suggest the brain is more buffered from extreme temperatures. Nevertheless, dopaminergic signaling in the brain co-varied with heat dissipation behaviors, providing a mechanism by which temporary thermal challenges may alter motivational circuits for song production. In both brain and testis, we also observed quantitative continuous variation between thermally sensitive gene networks and individual differences in thermoregulatory behavior, indicating that mechanisms of thermal-behavioral tolerance have microevolutionary potential. Taken together, these results suggest that selection for thermal tolerance could impact performance of sexually selected traits, and in turn, sexual selection in a warming world could influence how thermal tolerance evolves.
 
Overall design 2 tissues (posterior telencephalon and testis) were collected from 24 male zebra finches exposed to temperatures above (Ta = 43°C), within (Ta = 35°C), or below their thermoneutral zone (Ta = 27°C); total RNA was extracted and run on a Nextseq platform.
 
Contributor(s) Lipshutz SE, Howell CR, Buechlein AM, Rusch DB, Rosvall KA, Derryberry EP
Citation(s) 35567363
Submission date Oct 02, 2021
Last update date Jun 27, 2023
Contact name Kimberly A Rosvall
E-mail(s) krosvall@indiana.edu
Organization name Indiana University
Street address Dept of Biology, Indiana Univ., 1001 E. 3rd St, Jordan Hall
City Bloomington
State/province IN
ZIP/Postal code 47405
Country USA
 
Platforms (1)
GPL22780 Illumina NextSeq 500 (Taeniopygia guttata)
Samples (46)
GSM5607899 T142_27_PTR
GSM5607900 T143_27_PTR
GSM5607901 T145_27_PTR
Relations
BioProject PRJNA768106
SRA SRP339778

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE185196_ZF_PosteriorTelencephalon_GeneCounts.tsv.gz 634.9 Kb (ftp)(http) TSV
GSE185196_ZF_Testis_GeneCounts.tsv.gz 1.0 Mb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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