NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE185207 Query DataSets for GSE185207
Status Public on Oct 03, 2024
Title Immunogenic Senescence Sensitizes Lung Cancer to LUNX-Targeting Therapy [A549]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The higher immunogenicity of tumors usually predicts favorable therapeutic responses. Tumor antigens dominate the immunogenic character within tumors. We investigated if there was a targetable tumor antigen during immunogenic chemotherapy within lung cancer. Chemotherapy-induced immunogenic senescence was demonstrated using a multi-marker, three-step workflow and RNA-sequencing data. The ability of anti-lung-specific X protein (LUNX) antibody to suppress the survival of senescent lung cancer cells was evaluated in vitro and in vivo using real-time cytotoxicity analysis and xenograft mouse models, respectively. The induction of cellular senescence by immunogenic chemotherapy boosted cell-surface shuttling of LUNX and enhanced the immunogenic features of senescent tumor cells, which sensitized lung cancer cells to anti-LUNX antibody-mediated therapy and contributed to tumor suppression. The immunogenic senescence-mediated anti-tumor response was triggered by the direct action of antibody on tumor cells, strengthened by natural-killer cells through an antibody-dependent cell-mediated cytotoxicity response and, ultimately, led to tumor control. Our findings suggest that LUNX is a lung cancer targetable-immunogenic antigen. The proportion of lung cancers responding to LUNX-targeting therapy could be expanded substantially by immunogenic chemotherapy that induces senescence-associated translocation of LUNX to the plasma membrane.
 
Overall design Examination of the differences between DMSO-treated A549 cells and mitoxantrone-treated A549 cells
 
Contributor(s) Defeng J
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Oct 03, 2021
Last update date Oct 03, 2024
Contact name Defeng Jiao
E-mail(s) hly521@mail.ustc.edu.cn
Phone 15209882029
Organization name USTC
Street address 黄山路443号
City 合肥
ZIP/Postal code 230026
Country China
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM5608096 A549_DMSO [D1]
GSM5608097 A549_DMSO [D2]
GSM5608098 A549_DMSO [D3]
This SubSeries is part of SuperSeries:
GSE185209 Immunogenic Senescence Sensitizes Lung Cancer to LUNX-Targeting Therapy
Relations
BioProject PRJNA768289
SRA SRP340021

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE185207_A549-Group_D-VS-M_DE_significant_anno.txt.gz 825.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.