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Status |
Public on Oct 03, 2024 |
Title |
Immunogenic Senescence Sensitizes Lung Cancer to LUNX-Targeting Therapy [A549] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The higher immunogenicity of tumors usually predicts favorable therapeutic responses. Tumor antigens dominate the immunogenic character within tumors. We investigated if there was a targetable tumor antigen during immunogenic chemotherapy within lung cancer. Chemotherapy-induced immunogenic senescence was demonstrated using a multi-marker, three-step workflow and RNA-sequencing data. The ability of anti-lung-specific X protein (LUNX) antibody to suppress the survival of senescent lung cancer cells was evaluated in vitro and in vivo using real-time cytotoxicity analysis and xenograft mouse models, respectively. The induction of cellular senescence by immunogenic chemotherapy boosted cell-surface shuttling of LUNX and enhanced the immunogenic features of senescent tumor cells, which sensitized lung cancer cells to anti-LUNX antibody-mediated therapy and contributed to tumor suppression. The immunogenic senescence-mediated anti-tumor response was triggered by the direct action of antibody on tumor cells, strengthened by natural-killer cells through an antibody-dependent cell-mediated cytotoxicity response and, ultimately, led to tumor control. Our findings suggest that LUNX is a lung cancer targetable-immunogenic antigen. The proportion of lung cancers responding to LUNX-targeting therapy could be expanded substantially by immunogenic chemotherapy that induces senescence-associated translocation of LUNX to the plasma membrane.
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Overall design |
Examination of the differences between DMSO-treated A549 cells and mitoxantrone-treated A549 cells
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Contributor(s) |
Defeng J |
Citation missing |
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Submission date |
Oct 03, 2021 |
Last update date |
Oct 03, 2024 |
Contact name |
Defeng Jiao |
E-mail(s) |
hly521@mail.ustc.edu.cn
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Phone |
15209882029
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Organization name |
USTC
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Street address |
黄山路443号
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City |
合肥 |
ZIP/Postal code |
230026 |
Country |
China |
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Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE185209 |
Immunogenic Senescence Sensitizes Lung Cancer to LUNX-Targeting Therapy |
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Relations |
BioProject |
PRJNA768289 |
SRA |
SRP340021 |