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Status |
Public on Oct 01, 2023 |
Title |
Gene expression profile of human leukemic cell line treated with TLR1/2 agonist. |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Treatment of MV4;11 cells with CU-T12-9 increased ubiquitination of c-Myc compared to control and correspondingly resulted in reduced K148 acetylation. Chromatin immunoprecipitation coupled to high-throughput sequencing (Chip-Seq) revealed a significant loss of c-Myc occupancy in the genome following TLR1/2 activation with CU-T12-9. As expected, diminished c-Myc binding resulted in gene downregulation of well-establish c-Myc targets. These findings suggest that TLR-mediated K63-linked ubiquitination of c-Myc at K148 inhibits activation of c-Myc by interfering with its acetylation at the same residue.
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Overall design |
mRNA profiles of MV4;11 cells treated with CU-T12-9.
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Contributor(s) |
Muto T, Kwangmin C, Starczynowski DT |
Citation missing |
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Submission date |
Oct 04, 2021 |
Last update date |
Oct 01, 2023 |
Contact name |
Kwangmin Choi |
Organization name |
Cincinnati Children's Hospital Medical Center
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Department |
Experimental Hematology & Cancer Biology
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Street address |
333 Burnet Avenue
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City |
Cincinnati |
State/province |
Ohio |
ZIP/Postal code |
45229 |
Country |
USA |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (4)
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Relations |
BioProject |
PRJNA768468 |
SRA |
SRP339990 |