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Series GSE185299 Query DataSets for GSE185299
Status Public on Oct 01, 2023
Title Gene expression profile of human leukemic cell line treated with TLR1/2 agonist.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Treatment of MV4;11 cells with CU-T12-9 increased ubiquitination of c-Myc compared to control and correspondingly resulted in reduced K148 acetylation. Chromatin immunoprecipitation coupled to high-throughput sequencing (Chip-Seq) revealed a significant loss of c-Myc occupancy in the genome following TLR1/2 activation with CU-T12-9. As expected, diminished c-Myc binding resulted in gene downregulation of well-establish c-Myc targets. These findings suggest that TLR-mediated K63-linked ubiquitination of c-Myc at K148 inhibits activation of c-Myc by interfering with its acetylation at the same residue.
 
Overall design mRNA profiles of MV4;11 cells treated with CU-T12-9.
 
Contributor(s) Muto T, Kwangmin C, Starczynowski DT
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Submission date Oct 04, 2021
Last update date Oct 01, 2023
Contact name Kwangmin Choi
Organization name Cincinnati Children's Hospital Medical Center
Department Experimental Hematology & Cancer Biology
Street address 333 Burnet Avenue
City Cincinnati
State/province Ohio
ZIP/Postal code 45229
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (4)
GSM5610359 613MV411DMSO1
GSM5610360 614MV411DMSO2
GSM5610361 615MV411TLR1
Relations
BioProject PRJNA768468
SRA SRP339990

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE185299_GEAPTbox_MV411_DMSO_and_TLR_matrix.csv.gz 393.7 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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