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| Status |
Public on Mar 31, 2022 |
| Title |
Chromatin reorganization during myoblast differentiation involves the caspase-dependent removal of SATB2 [Hi-C] |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Induction of lineage-specific gene programs are strongly influenced by alterations in local chromatin architecture. However, key players that impact this genome reorganization remain largely unknown. Here, we report that removal of special AT-rich binding protein 2 (SATB2), a nuclear protein known to bind matrix attachment regions, is a key event in initiating myogenic differentiation. Deletion of myoblast SATB2 in vitro initiates chromatin remodeling and accelerates differentiation, while in vivo ablation depletes the muscle progenitor pool. Genome wide analysis indicates that SATB2 binding influences both chromatin loop and sub-TAD domain formation. These chromatin changes are both repressive and inductive, as loss of SATB2 leads to expression of differentiation regulatory factors and inhibition of genes that impair this process. Finally, we noted that the differentiation-specific decline in SATB2 protein is dependent on a caspase 7-mediated cleavage event. Taken together, this study demonstrates that temporal control of SATB2 protein is critical for shaping the chromatin environment and coordinating the myogenic differentiation program
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| |
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| Overall design |
Hi-C of siControl and siSatb2 proliferating C2C12 cells (2 replicates)
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| Contributor(s) |
Bell RA, Bandukwala H, Megeney LA |
| Citation(s) |
35326417 |
| |
| Submission date |
Oct 06, 2021 |
| Last update date |
Mar 31, 2022 |
| Contact name |
Lynn Megeney |
| E-mail(s) |
lmegeney@ohri.ca
|
| Phone |
6137378618
|
| Organization name |
Ottawa Hospital Research Institute
|
| Department |
Regenerative Medicine Program
|
| Street address |
501 Smyth Road
|
| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
KIH 8L6 |
| Country |
Canada |
| |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE185437 |
Chromatin reorganization during myoblast differentiation involves the caspase-dependent removal of SATB2 |
|
| Relations |
| BioProject |
PRJNA769206 |
| SRA |
SRP340441 |
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