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| Status |
Public on Jun 20, 2022 |
| Title |
Single-nucleus chromatin accessibility and RNA-seq reveal impaired brain development in prenatal e-cigarette exposed neonatal rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We studied the influence of maternal vaping on rat neonates' brain development, using single nucleus -ATAC-seq (snATAC-seq) and -RNA-seq (snRNA-seq) technologies. We found that maternal vaping distorted neuronal lineage differentiation by promoting excitatory neuron and inhibiting lateral ganglionic eminence derived inhibitory neuron differentiation. Maternal vaping also diminished microglia population in rat developing brain. Functional enrichment revealed that the distorted neuronal differentiation and reduced microglia population were associated with disrupted brain calcium homeostasis and signaling. Our findings raise the concern that maternal vaping may cause adverse long-term brain damage to the offsprings. In addition, we proposed a set of brain cell specific chromatin accessibility markers for identifying rat brain cells directly from snATAC-seq data.
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| Overall design |
Pregnant rats were exposed to e-cigarette vapor during E4 to E20. Droplet based snATAC-seq and snRNA-seq were performed to study the influence of maternal vaping on neonates' brain development.
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| Contributor(s) |
Wang C, Xiao D |
| Citation(s) |
35874099 |
| |
| Submission date |
Oct 07, 2021 |
| Last update date |
Aug 03, 2022 |
| Contact name |
Charles Wang |
| E-mail(s) |
chwang@llu.edu
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| Phone |
(909) 558-4325
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| Organization name |
Loma Linda University
|
| Department |
Department of Basic Sciences
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| Lab |
Center for Genomics
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| Street address |
11021 Campus Street
|
| City |
Loma Linda |
| State/province |
CA |
| ZIP/Postal code |
92350 |
| Country |
USA |
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| Platforms (1) |
| GPL22396 |
Illumina HiSeq 4000 (Rattus norvegicus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA769452 |
| SRA |
SRP340445 |
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