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Series GSE185633 Query DataSets for GSE185633
Status Public on Oct 20, 2023
Title NQO1 regulates expression and alternative splicing of apoptotic genes associated with Alzheimer's disease in PC-12 cells
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Quinone oxidoreductase I (NQO1), an important antioxidant enzyme which plays an important role in monitoring cellular redox state, was altered in the brain tissues of Alzheimer’s disease (AD) patients. In addition to its traditional antioxidant effect, NQO1 is also a multifunctional RNA-binding protein (RBP) in post-transcriptional regulation. However, NQO1 in AD pathology by acting as an RBP is not studied. In the present study, the RBP functions of NQO1 in rat PC12 cells, a cell line widely used in neurological disease studies, were investigated using siRNA knock-down (KD) and following whole transcriptome (RNA-seq) analysis. Reduced levels of NQO1 led to a significant increase in cellular apoptosis, compared with control cells. Notably, RNA-seq analysis of the transcriptome of PC12 cells by NQO1-KD revealed that genes in certain apoptosis pathways, were under global transcriptional and alternative splicing regulation. Quantitative RT-PCR confirmed the NQO1-regulated transcription of apoptotic genes Cryab, Lgmn , Ngf, Apoe, Brd7, Stat3, and alternative splicing of Bin1, Picalm, Fyn. These NQO1-regulated genes have been found to be closely related to AD pathogenesis. Our findings suggest that NQO1 acts as an RBP and participates in the pathology of AD by regulating expression and alternative splicing of genes involved in apoptosis. The results of present study extend our understanding of the cellular and molecular mechanisms in AD pathogenesis, which might contribute to the development of novel therapeutic targets.
 
Overall design Transcrptional analysis of nqo1 knockdown and control in PC12 cells
 
Contributor(s) Du Y, Liu G, Liu Y
Citation(s) 37002649
Submission date Oct 11, 2021
Last update date Oct 20, 2023
Contact name yingshi du
E-mail(s) duyingshi2013@126.com
Phone 86-15693052696
Organization name The First Hospital of Lanzhou University
Department First Department of Geriatrics
Street address NO.1 Donggang West Road
City LanZhou
ZIP/Postal code 730000
Country China
 
Platforms (1)
GPL24688 HiSeq X Ten (Rattus norvegicus)
Samples (6)
GSM5620968 shNQO1_1st
GSM5620969 shNQO1_2st
GSM5620970 shNQO1_3st
Relations
BioProject PRJNA770248
SRA SRP340811

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE185633_expressed_gene_FPKM.txt.gz 630.6 Kb (ftp)(http) TXT
GSE185633_expressed_gene_reads.txt.gz 596.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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