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| Status |
Public on Oct 15, 2021 |
| Title |
Single-cell RNA-Sequencing maps the hematopoietic landscape in naked mole-rats [unfractionated BM] |
| Organisms |
Mus musculus; Heterocephalus glaber |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Naked mole-rats are a mammalian model organism of exceptional longevity. We mapped the hematopoietic hierarchy and used functional characterizations to define a purified stem and progenitor cell (HSPC) compartment similar to mouse LIN-/Sca-1+/Kit+ HSPCs or human LIN-/CD34+/CD38lo HSPCs.
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| Overall design |
Naked mole-rat or Mouse bone marrow was extracted, RBC-lysed and submitted to scRNA-Seq as unfractionated BM, in parallel FACS-antibodies were conjugated to oligo-ADT tags to quantify surface marker binding as described for CITE-Seq. The mRNA libraries were aligned to either the FRAMA bone marrow transcriptome assembly from our publication or a mouse genome reference.
Note: Raw data represents only gene expresion (GEX). Raw data for ADT not included.
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| Contributor(s) |
Emmrich S |
| Citation(s) |
34596321 |
| Submission date |
Oct 12, 2021 |
| Last update date |
May 26, 2022 |
| Contact name |
Stephan Emmrich |
| E-mail(s) |
stephan.emmrich@rochester.edu
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| Organization name |
University of Rochester
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| Street address |
213 Hutchison Hall, River Campus
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| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14627 |
| Country |
USA |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL30845 |
Illumina NovaSeq 6000 (Heterocephalus glaber) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE185724 |
Single-cell RNA-Sequencing maps the hematopoietic landscape in naked mole-rats. |
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| Relations |
| BioProject |
PRJNA770625 |
| SRA |
SRP341003 |
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