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Status |
Public on Oct 15, 2021 |
Title |
Single-cell RNA-Sequencing maps the hematopoietic landscape in naked mole-rats [unfractionated LY] |
Organisms |
Mus musculus; Heterocephalus glaber |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Naked mole-rats are a mammalian model organism of exceptional longevity. We mapped the T cell developmental landscape in Naked mole-rats and showed
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Overall design |
Naked mole-rat or Mouse lymphatic tissues were extracted, RBC-lysed and submitted to scRNA-Seq as unfractionated thymus or lymph nodes, in parallel FACS-antibodies were conjugated to oligo-ADT tags to quantify surface marker binding as described for CITE-Seq. The mRNA libraries were aligned to either the FRAMA bone marrow transcriptome assembly from our publication or a mouse genome reference.
Note: Raw data represents only gene expresion (GEX). Raw data for ADT not included.
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Contributor(s) |
Emmrich S |
Citation(s) |
- Emmrich S, Tolibzoda Zakusilo F, Trapp A, Zhou X et al. Ectopic cervical thymi and no thymic involution until midlife in naked mole rats. Aging Cell 2021 Oct;20(10):e13477. PMID: 34596321
- Sanchez Sanchez G, Emmrich S, Georga M, Papadaki A et al. Invariant γδTCR natural killer-like effector T cells in the naked mole-rat. Nat Commun 2024 May 18;15(1):4248. PMID: 38762584
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Submission date |
Oct 12, 2021 |
Last update date |
Jun 06, 2024 |
Contact name |
Stephan Emmrich |
E-mail(s) |
stephan.emmrich@rochester.edu
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Organization name |
University of Rochester
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Street address |
213 Hutchison Hall, River Campus
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City |
Rochester |
State/province |
NY |
ZIP/Postal code |
14627 |
Country |
USA |
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Platforms (2) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
GPL30845 |
Illumina NovaSeq 6000 (Heterocephalus glaber) |
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Samples (14)
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This SubSeries is part of SuperSeries: |
GSE185724 |
Single-cell RNA-Sequencing maps the hematopoietic landscape in naked mole-rats. |
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Relations |
BioProject |
PRJNA770624 |
SRA |
SRP341004 |