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Series GSE185721 Query DataSets for GSE185721
Status Public on Oct 15, 2021
Title Single-cell RNA-Sequencing maps the hematopoietic landscape in naked mole-rats [unfractionated LY]
Organisms Mus musculus; Heterocephalus glaber
Experiment type Expression profiling by high throughput sequencing
Summary Naked mole-rats are a mammalian model organism of exceptional longevity. We mapped the T cell developmental landscape in Naked mole-rats and showed
 
Overall design Naked mole-rat or Mouse lymphatic tissues were extracted, RBC-lysed and submitted to scRNA-Seq as unfractionated thymus or lymph nodes, in parallel FACS-antibodies were conjugated to oligo-ADT tags to quantify surface marker binding as described for CITE-Seq. The mRNA libraries were aligned to either the FRAMA bone marrow transcriptome assembly from our publication or a mouse genome reference.

Note: Raw data represents only gene expresion (GEX). Raw data for ADT not included.
 
Contributor(s) Emmrich S
Citation(s)
  • Emmrich S, Tolibzoda Zakusilo F, Trapp A, Zhou X et al. Ectopic cervical thymi and no thymic involution until midlife in naked mole rats. Aging Cell 2021 Oct;20(10):e13477. PMID: 34596321
  • Sanchez Sanchez G, Emmrich S, Georga M, Papadaki A et al. Invariant γδTCR natural killer-like effector T cells in the naked mole-rat. Nat Commun 2024 May 18;15(1):4248. PMID: 38762584
Submission date Oct 12, 2021
Last update date Jun 06, 2024
Contact name Stephan Emmrich
E-mail(s) stephan.emmrich@rochester.edu
Organization name University of Rochester
Street address 213 Hutchison Hall, River Campus
City Rochester
State/province NY
ZIP/Postal code 14627
Country USA
 
Platforms (2)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL30845 Illumina NovaSeq 6000 (Heterocephalus glaber)
Samples (14)
GSM5622049 113_hglBLN1
GSM5622050 113_hglTHY1
GSM5622051 114_hglTHY2
This SubSeries is part of SuperSeries:
GSE185724 Single-cell RNA-Sequencing maps the hematopoietic landscape in naked mole-rats.
Relations
BioProject PRJNA770624
SRA SRP341004

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE185721_molecules.hglLY.uniqe_adtcounts_anno.RData.gz 215.6 Mb (ftp)(http) RDATA
GSE185721_molecules.mmuTHY.uniqe_adtcounts_anno.RData.gz 193.8 Mb (ftp)(http) RDATA
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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