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Status |
Public on Apr 15, 2010 |
Title |
Genome-wide maps of CFP1, RNA Polymerase II and H3K4me3 in mouse brain. |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We report the application of single molecule-based sequencing technology for high-throughput mapping of CFP1, RNA polymerase II and H3K4me3 in mouse brain. By obtaining sequence from chromatin immunoprecipitated DNA, we generated genome-wide binding / chromatin-state maps for mouse brain. We find a good correlation between CFP1 binding and H3K4me3 consistent with it presence in the SetD1 histone methylatransferase complex. Mapped RNA polymerase II colocalised with the majority of CFP1 / H3K4me3 positive CpG islands but not all. This study provides a comprehensive characterisation of the genome wide distribution of a previously uncharacaterised DNA binding factor and suggests a link between DNA base composition and chromatin state.
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Overall design |
Examination of H3K4me3, RNA PolymeraseII and CFP1 in mouse brain.
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Contributor(s) |
Thomson JP, Skene PJ, Selfridge J, Guy J, Deaton A, Kerr A, Webb S, Andrews R, James KD, Turner DJ, McLaren S, Illingworth RS, Bird AP |
Citation(s) |
20393567 |
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Submission date |
Oct 15, 2009 |
Last update date |
May 15, 2019 |
Contact name |
Adrian P Bird |
E-mail(s) |
A.Bird@ed.ac.uk
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Phone |
0131 6508695
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Fax |
0131 6505379
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Organization name |
The University of Edinburgh
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Department |
WTCCB
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Lab |
Bird Lab
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Street address |
Mayfield Road
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City |
Edinburgh |
State/province |
Midlothian |
ZIP/Postal code |
EH9 3JR |
Country |
United Kingdom |
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Platforms (1) |
GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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Samples (3) |
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Relations |
SRA |
SRP002011 |
BioProject |
PRJNA120285 |
Supplementary file |
Size |
Download |
File type/resource |
GSE18578_RAW.tar |
200.7 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
Processed data provided as supplementary file |
Raw data are available in SRA |
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