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Series GSE18578 Query DataSets for GSE18578
Status Public on Apr 15, 2010
Title Genome-wide maps of CFP1, RNA Polymerase II and H3K4me3 in mouse brain.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report the application of single molecule-based sequencing technology for high-throughput mapping of CFP1, RNA polymerase II and H3K4me3 in mouse brain. By obtaining sequence from chromatin immunoprecipitated DNA, we generated genome-wide binding / chromatin-state maps for mouse brain. We find a good correlation between CFP1 binding and H3K4me3 consistent with it presence in the SetD1 histone methylatransferase complex. Mapped RNA polymerase II colocalised with the majority of CFP1 / H3K4me3 positive CpG islands but not all. This study provides a comprehensive characterisation of the genome wide distribution of a previously uncharacaterised DNA binding factor and suggests a link between DNA base composition and chromatin state.
 
Overall design Examination of H3K4me3, RNA PolymeraseII and CFP1 in mouse brain.
 
Contributor(s) Thomson JP, Skene PJ, Selfridge J, Guy J, Deaton A, Kerr A, Webb S, Andrews R, James KD, Turner DJ, McLaren S, Illingworth RS, Bird AP
Citation(s) 20393567
Submission date Oct 15, 2009
Last update date May 15, 2019
Contact name Adrian P Bird
E-mail(s) A.Bird@ed.ac.uk
Phone 0131 6508695
Fax 0131 6505379
Organization name The University of Edinburgh
Department WTCCB
Lab Bird Lab
Street address Mayfield Road
City Edinburgh
State/province Midlothian
ZIP/Postal code EH9 3JR
Country United Kingdom
 
Platforms (1)
GPL9185 Illumina Genome Analyzer (Mus musculus)
Samples (3)
GSM462190 RNAPII
GSM462191 CFP1
GSM462192 H3K4me3
Relations
SRA SRP002011
BioProject PRJNA120285

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Supplementary file Size Download File type/resource
GSE18578_RAW.tar 200.7 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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