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| Status |
Public on Oct 22, 2021 |
| Title |
A pigtailed macaque model for Kyasanur Forest disease virus and Alkhurma hemorrhagic disease virus pathogenesis |
| Organism |
Macaca nemestrina |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Kyasanur Forest disease virus (KFDV) and the closely related Alkhurma hemorrhagic disease virus (AHFV) are emerging flaviviruses that cause severe viral hemorrhagic fevers in humans. Increasing geographical expansion and case numbers, particularly of KFDV in southwest India, class these viruses as a public health threat. Viral pathogenesis is not well understood and additional vaccines and antivirals are needed to effectively counter the impact of these viruses. However, current animal models for KFDV do not accurately reproduce viral tissue tropism or clinical outcomes observed in humans. Here, we show pigtailed macaques (Macaca nemestrina) infected with KFDV or AHFV develop viremia that peaks 2 to 4 days following inoculation. Over the course of infection, animals developed lymphocytopenia, thrombocytopenia, and elevated liver enzymes. Infected animals exhibited hallmark signs of human disease characterized by a flushed appearance, piloerection, dehydration, loss of appetite, weakness, and hemorrhagic signs such as epistaxis. Virus was commonly present in the gastrointestinal tract, consistent with human disease caused by KFDV and AHFV where gastrointestinal symptoms (hemorrhage, vomiting, diarrhea) are common. This work characterizes a nonhuman primate model for KFDV and AHFV that closely resembles human disease for further utilization in understanding host immunity and development of antiviral countermeasures.
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| Overall design |
pigtailed macaque model for Kyasanur Forest disease virus and Alkhurma hemorrhagic disease virus pathogenesis
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| Contributor(s) |
Broeckel RM, Feldmann F, McNally KL, Chiramel AI, Sturdevant G, Leung J, Hanley PW, Lovaglio J, Rosenke R, Scott DP, Saturday G, Bouamr F, Rasmussen A, Robertson SJ, Best SM |
| Citation missing |
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| Submission date |
Oct 13, 2021 |
| Last update date |
Oct 22, 2021 |
| Contact name |
Dan Sturdevant |
| E-mail(s) |
dsturdevant@niaid.nih.gov
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| Phone |
4063639248
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| Organization name |
NIH
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| Department |
NIAID
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| Lab |
RTS
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| Street address |
903 S 4th street
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| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
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| Platforms (1) |
| GPL30848 |
NextSeq 550 (Macaca nemestrina) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA770874 |
| SRA |
SRP341150 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE185797_matrix_mmul_raw.txt.gz |
829.7 Kb |
(ftp)(http) |
TXT |
| GSE185797_matrix_normalized_counts.txt.gz |
2.9 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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