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Status |
Public on Apr 13, 2022 |
Title |
ALKBH5 promotes tumor progression by decreasing RIG-I expression mediated by N6-methyladenosine-dependent HNRNPC binding in HNSCC [MeRIP-seq] |
Organism |
Homo sapiens |
Experiment type |
Methylation profiling by high throughput sequencing Expression profiling by high throughput sequencing
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Summary |
To determine the targets underlying ALKBH5 during head and neck squamouse cell carcinoma progression, Methylated RNA immunoprecipitation (MeRIP) with an m6A specific antibody followed by RNA sequencing (MeRIP-seq) and next generation sequencing were combined to screen the potential targets haboring m6A modificatios and mRNA level alteration after ALKBH5 knockdown in a HNSCC cell line.
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Overall design |
MeRIP-seq was conducted in Cal27 cells after ALKBH5 silencing for 24 h.
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Contributor(s) |
Ma H, Jin S |
Citation(s) |
35395767 |
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Submission date |
Oct 13, 2021 |
Last update date |
Apr 13, 2022 |
Contact name |
Hailong Ma |
E-mail(s) |
mahl21@sjtu.edu.cn
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Organization name |
Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine
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Street address |
Zhizaoju Rd
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City |
Shanghai |
ZIP/Postal code |
200011 |
Country |
China |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (8)
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This SubSeries is part of SuperSeries: |
GSE185888 |
ALKBH5 promotes tumor progression by decreasing RIG-I expression mediated by N6-methyladenosine-dependent HNRNPC binding in HNSCC |
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Relations |
BioProject |
PRJNA771104 |
SRA |
SRP341304 |