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| Status |
Public on Aug 08, 2023 |
| Title |
Effects of culture condition on small RNA transcriptomes in extracellular vesicles released by human bone marrow stromal cells |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Various culture media that can rapidly expand bone marrow stromal cells (BMSCs) are currently available. However, the effects of those culture media on the contents of extracellular vesicles released by bone marrow stromal cells have not been fully understood. Using BMSCs from 6 healthy donors were cultured in two different culture media and characterized the small RNA profiles in extracellular vesicles.
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| Overall design |
Total 12 samples were analyzed; BMSCs (#3-#8) were cultured in either DMEM+10% FBS (D) or high perfomance medium (R)
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| Contributor(s) |
Scheiber AL, Clark CA, Kaito T, Iwamoto M, Horwitz EM, Kawasawa YI, Otsuru S |
| Citation(s) |
35162938 |
| |
| Submission date |
Oct 14, 2021 |
| Last update date |
Aug 08, 2023 |
| Contact name |
Yuka Imamura Kawasawa |
| E-mail(s) |
yui102@psu.edu
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| Organization name |
Penn State University
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| Department |
College of Medicine, Pharmacology
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| Street address |
500 University Dr.
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| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA771408 |
| SRA |
SRP341464 |
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