NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE186068 Query DataSets for GSE186068
Status Public on Mar 11, 2022
Title Systematic reconstruction of the cellular trajectories of mammalian embryogenesis (deeper sequencing of E9.5-E13.5)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Mammalian embryogenesis is characterized by rapid cellular proliferation and diversification. Within a few weeks, a single cell zygote gives rise to millions of cells expressing a panoply of molecular programs, including much of the diversity that will subsequently be present in adult tissues. Although intensively studied, a comprehensive delineation of the major cellular trajectories that comprise mammalian development in vivo remains elusive.
For mouse embryogenesis in particular, we and others have performed single cell or single nucleus RNA-seq data (scRNA-seq) during implantation, gastrulation and organogenesis. Here we set out to integrate several single cell RNA-seq datasets (scRNA-seq) that collectively span mouse gastrulation and organogenesis. However, a technical challenge that we faced is that the datasets that we sought to integrate were generated by different groups at different times using different scRNA-seq technologies. In particular, probably because there was no overlapping timepoint, the integration of scRNA-seq data generated at E8.5 (cells, 10X Genomics) and E9.5 (nuclei, sci-RNA-seq3) was challenging (Cao et al. 2019; Pijuan-Sala et al. 2019).
To address this, we set out to generate new data at E8.5 that might serve to “bridge” these two datasets. Because of how quickly changes are occurring during this window of development, we focused on individual, somite-resolved E8.5 embryos using a simplified, optimized version of sci-RNA-seq3. At the same time, to obtain higher quality data across E9.5-E13.5, we performed a deeper sequencing (specifically, three additional Novaseq runs) of previously reported libraries (Cao et al. 2019). Compared to the previous data (Cao et al. 2019), the median UMI count per cell improved from 671 to 1,434, while the median genes detected per cell improved from 518 to 735.
Overall, we collected published data (Cheng et al. 2019; Mohammed et al. 2017; Pijuan-Sala et al. 2019), the new E8.5 data, and the deeper sequencing of previous libraries (Cao et al. 2019). Altogether, we define cell states at each of 19 successive stages spanning E3.5 to E13.5, heuristically connect them to their pseudo-ancestors and pseudo-descendants. Despite being constructed through automated procedures, the resulting trajectories of mammalian embryogenesis (TOME) are largely consistent with our contemporary understanding of mammalian development.
In addition, the data of deeper sequencing of previously reported libraries itself comprises a foundational resource for mammalian developmental biology, and are made available in a way that will facilitate their ongoing annotation by the research community.
 
Overall design 2 million cells derived from 61 mouse embryos staged between 9.5 and 13.5 days of gestation (E9.5 to E13.5; 10 to 15 replicates per timepoint)
 
Contributor(s) Qiu C, Cao J, Martin B, Li T, Welsh IC, Srivatsan S, Huang X, Calderon D, Noble WS, Disteche CM, Murray SA, Spielmann M, Moens CB, Trapnell C, Shendure J
Citation(s) 35288709, 38355799
Submission date Oct 18, 2021
Last update date Mar 06, 2024
Contact name Chengxiang Qiu
E-mail(s) cxqiu@uw.edu
Organization name University of Washington
Department Genome Sciences
Lab Jay Shendure
Street address William H. Foege Hall, 3720 15th Ave NE
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (760)
GSM5630100 sci-RNA-seq3 with mouse embryo atlas (deeper sequencing) sci3-me-001
GSM5630101 sci-RNA-seq3 with mouse embryo atlas (deeper sequencing) sci3-me-002
GSM5630102 sci-RNA-seq3 with mouse embryo atlas (deeper sequencing) sci3-me-003
This SubSeries is part of SuperSeries:
GSE186070 Systematic reconstruction of the cellular trajectories of mammalian embryogenesis
Relations
BioProject PRJNA772275
SRA SRP341943

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE186068_cell_annotate.csv.gz 46.6 Mb (ftp)(http) CSV
GSE186068_gene_annotate.csv.gz 943.7 Kb (ftp)(http) CSV
GSE186068_gene_count.loom.gz 8.4 Gb (ftp)(http) LOOM
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap