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Status |
Public on Oct 16, 2023 |
Title |
Human monocyte-derived macrophages polarized by M-CSF in the presence or absence of 5 micromolar Methotrexate |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Macrophage phenotypic and functional heterogeneity derives from tissue-specific transcriptional signatures shaped by the local microenvironment. M-CSF drives the generation of human monocyte-derived macrophages with a potent anti-inflammatory activity upon stimulation. One-carbon metabolism (OCM) is a complex network of biosynthetic pathways that includes de novo biosynthesis of purines and thymidylate, amino acid metabolism, and methylation reactions. We explored the molecular impact of blocking OCM with high-doses of the anti-folate methotrexate (MTX) on the gene expression profile in M-CSF-primed human monocyte derived macrophages.
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Overall design |
mRNA profiles of human macrophages differentiated with M-CSF (M-MØ) from peripheral blood monocytes in the absence or in the presence of high doses of the antifolate methotrexate (MTX). MTX (5 uM) was added once on monocytes inmediatly before the initation of the M-CSF differentiation process.
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Contributor(s) |
Lorente-Sorolla C, Torres Torresano M, Puig Kröger A |
Citation(s) |
36380627 |
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Submission date |
Oct 19, 2021 |
Last update date |
Jan 15, 2024 |
Contact name |
Amaya Rocío Puig Kröger |
E-mail(s) |
amaya.puig@iisgm.com
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Phone |
+34 914269268
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Organization name |
Hospital General Universitario Gregorio Marañón
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Department |
Immunology
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Lab |
Immunometabolism and Infammation
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Street address |
Dr. Esquerdo 46
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City |
Madrid |
State/province |
Madrid |
ZIP/Postal code |
28007 |
Country |
Spain |
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Platforms (1) |
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Samples (6)
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GSM5639440 |
M2_MTX_A: MTX-M-MØ macrophage donor1 rep1 |
GSM5639441 |
M2_MTX_B: MTX-M-MØ macrophage donor2 rep2 |
GSM5639442 |
M2_MTX_C: MTX-M-MØ macrophage donor3 rep3 |
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Relations |
BioProject |
PRJNA772745 |
SRA |
SRP342152 |