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Series GSE186151 Query DataSets for GSE186151
Status Public on Oct 16, 2023
Title Human monocyte-derived macrophages polarized by M-CSF in the presence or absence of 5 micromolar Methotrexate
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Macrophage phenotypic and functional heterogeneity derives from tissue-specific transcriptional signatures shaped by the local microenvironment. M-CSF drives the generation of human monocyte-derived macrophages with a potent anti-inflammatory activity upon stimulation. One-carbon metabolism (OCM) is a complex network of biosynthetic pathways that includes de novo biosynthesis of purines and thymidylate, amino acid metabolism, and methylation reactions. We explored the molecular impact of blocking OCM with high-doses of the anti-folate methotrexate (MTX) on the gene expression profile in M-CSF-primed human monocyte derived macrophages.
 
Overall design mRNA profiles of human macrophages differentiated with M-CSF (M-MØ) from peripheral blood monocytes in the absence or in the presence of high doses of the antifolate methotrexate (MTX). MTX (5 uM) was added once on monocytes inmediatly before the initation of the M-CSF differentiation process.
 
Contributor(s) Lorente-Sorolla C, Torres Torresano M, Puig Kröger A
Citation(s) 36380627
Submission date Oct 19, 2021
Last update date Jan 15, 2024
Contact name Amaya Rocío Puig Kröger
E-mail(s) amaya.puig@iisgm.com
Phone +34 914269268
Organization name Hospital General Universitario Gregorio Marañón
Department Immunology
Lab Immunometabolism and Infammation
Street address Dr. Esquerdo 46
City Madrid
State/province Madrid
ZIP/Postal code 28007
Country Spain
 
Platforms (1)
GPL23227 BGISEQ-500 (Homo sapiens)
Samples (6)
GSM5639437 M2_A: M-MØ macrophage donor1 rep1
GSM5639438 M2_B: M-MØ macrophage donor2 rep2
GSM5639439 M2_C: M-MØ macrophage donor3 rep3
Relations
BioProject PRJNA772745
SRA SRP342152

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE186151_RAW.tar 510.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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