|
| Status |
Public on Oct 28, 2009 |
| Title |
Early drought-responsive changes in pepper leaves and roots |
| Platform organisms |
Populus trichocarpa x Populus deltoides; Capsicum annuum; Solanum tuberosum; Ipomoea batatas; Triticum aestivum; Zea mays; Homo sapiens; Pinus pinaster; Populus euphratica; Populus tremula x Populus alba |
| Sample organism |
Capsicum annuum |
| Experiment type |
Expression profiling by array
|
| Summary |
Drought is one of the major factor that limits crop production and reduces yield. To understand the early response of plants under nearly natural conditions, pepper plants were grown in a greenhouse and drought stressed by withholding water for one week. Plants adapted to the decreasing water content of the substrate by adjustment of their osmotic potential in roots by accumulation of raffinose, glucose, galactinol and proline. In contrast in leaves levels of fructose, sucrose and also galactinol increased. Due to the water deficit cadaverine, putrescine, spermidine and spermine accumulated in leaves whereas the concentration of polyamines was reduced in roots. These polyamines are suggested to rather act as stress protectants than for osmotic adjustment. To understand the molecular basis of the response to this early drought stress better, four suppression subtractive hybridisation libraries from leaves and roots were constructed. Microarray technique was used to identify differentially expressed genes. A total of 109 unique ESTs were detected. The diversity of the putative functions of all identified genes confirms the complexity of the plant response to drought stress.
Keywords: Transcription profiling
|
| |
|
| Overall design |
Two-condition experiment in roots and leaves, control leaves (CL) vs. drought-stressed leaves (DL) and control roots (CR) vs. drought-stressed roots (DR). Biological replicates: 4 control (1-4), drought-stressed (1-4), independently grown and harvested. One swap replicate per array.
|
| |
|
| Contributor(s) |
Sziderics AH, Oufir M, Trognitz F, Matusikova I, Hausmann JF, Wilhelm E |
| Citation(s) |
20087595 |
| |
| Submission date |
Oct 19, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Silvia Fluch |
| Organization name |
Austrian Research Centers GmbH
|
| Department |
Biogenetics/Natural Resources
|
| Lab |
PICME
|
| Street address |
Campus Seibersdorf
|
| City |
Seibersdorf |
| ZIP/Postal code |
2444 |
| Country |
Austria |
| |
|
| Platforms (1) |
|
| Samples (8)
|
| GSM462967 |
Leaf_Control vs Drought Stressed_Replicate 1 |
| GSM462968 |
Leaf_Control vs Drought Stressed_Replicate 2 |
| GSM462969 |
Leaf_Control vs Drought Stressed_Replicate 3_Swap |
| GSM462970 |
Leaf_Control vs Drought Stressed_Replicate 4_Swap |
| GSM462971 |
Roots_Control vs Drought Stressed_Replicate 1 |
| GSM462972 |
Roots_Control vs Drought Stressed_Replicate 2 |
| GSM462973 |
Roots_Control vs Drought Stressed_Replicate 3_Swap |
| GSM462974 |
Roots_Control vs Drought Stressed_Replicate 4_Swap |
|
| Relations |
| BioProject |
PRJNA121467 |
Less...
More...