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Series GSE186502 Query DataSets for GSE186502
Status Public on Mar 31, 2023
Title The ASC-1 complex promotes translation initiation of the scanning ribosomes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Other
Summary Translation is initiated by binding of the eIF4F complex to the 5' cap of the mRNA, which is followed by scanning of the initiation codon by scanning ribosomes. Here we demonstrate that the ASC-1 complex (ASCC), which was previously shown to promote the dissociation of colliding 80S ribosomes, associates with the scanning ribosomes to regulate translation initiation. Sel-TCP-seq analysis revealed that ASCC3, a subunit of ASCC with a helicase domain, localizes predominantly to the 5' untranslated region of mRNAs. Knockdown of ASCC3 resulted in reduced translation efficiency associated with reduced 43S preinitiation complex (PIC) loading and a reduced speed of scanning ribosomes. In addition, depletion of the ubiquitin ligase ZNF598, a sensor of collided 80S ribosomes, also reduces the PIC loading and speed of scanning ribosomes. Our results have thus revealed that ASCC is required not only for dissociation of colliding 80S ribosomes, but also for efficient translation initiation by scanning ribosomes.
 
Overall design Translational dynamics regulated by eIF4F or ASC-1 complex
 
Contributor(s) Kito Y, Ichihara K, Matsumoto A, Nakayama K
Citation(s) 37092320
Submission date Oct 25, 2021
Last update date Apr 27, 2023
Contact name Kazuya Ichihara
E-mail(s) ichihara.kazuya.d5@f.mail.nagoya-u.ac.jp
Organization name Kyushu University
Street address 3-1-1, Maidashi
City Higashi-ku, Fukuoka
ZIP/Postal code 812-8582
Country Japan
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (74)
GSM5653549 siControl HEK293T RNA-seq replicate 1
GSM5653550 siControl HEK293T RNA-seq replicate 2
GSM5653551 eIF4A1 and eIF4A2 DKD HEK293T #1 RNA-seq replicate 1
Relations
BioProject PRJNA774189
SRA SRP342982

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE186502_Counts_Table.csv.gz 17.6 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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