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Series GSE186504 Query DataSets for GSE186504
Status Public on Oct 26, 2021
Title Interactions between different maturation states of oocytes and granulosa cells in human antral follicles identified by single-cell transcriptomics
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human ovarian folliculogenesis is an highly regulated and complex process. Characterization of follicular cell signatures during this dynamic process is important to understand follicle fate (to grow, become dominant or undergo atresia). The transcriptional signature of human oocytes and granulosa cells (GC) in early-growing and ovulatory follicles have been previously described, however that of oocytes with surrounding GCs in small antral follicles have not been studied yet. Here, we have generated a unique dataset of single-cell transcriptomics (SmartSeq2) consisting of the oocyte with surrounding GCs from several individual (non-dominant) small antral follicles isolated from adult human ovaries. We have identified two main types of (healthy) follicles, with a distinct oocyte and GC signature. Using the CellphoneDB algorithm, we then investigated the bi-directional ligand-receptor interactions regarding the TGFβ/BMP, WNT, NOTCH, and receptor tyrosine kinases (RTK) signaling pathways between oocyte and GCs within each antral follicle type. Our work not only revealed the diversity of small antral follicles but also contributes to fill the gap in mapping the molecular landscape of human folliculogenesis and oogenesis.
 
Overall design 141 cells from 14 individual antral follicles from 3 donor ovaries
 
Contributor(s) Chuva de Sousa Lopes SM, Moustakas I, Fan X
Citation(s) 34769386
Submission date Oct 25, 2021
Last update date Jan 27, 2022
Contact name Susana Chuva de Sousa Lopes
E-mail(s) S.M.Chuva_de_Sousa_Lopes@lumc.nl
Organization name Leiden University Medical Center
Department Anatomy and Embryology
Lab Chuva Sousa Lopes Lab
Street address Einthovenweg 20
City Leiden
ZIP/Postal code 2333ZC
Country Netherlands
 
Platforms (2)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (141)
GSM5653653 Cell from adult ovary follicle, S_A3
GSM5653654 Cell from adult ovary follicle, S_A1
GSM5653655 Cell from adult ovary follicle, S_A7
Relations
BioProject PRJNA774191
SRA SRP342988

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE186504_raw_counts.tsv.gz 2.0 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record
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