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Series GSE186615 Query DataSets for GSE186615
Status Public on Feb 13, 2022
Title Loss of m6A methyltransferase METTL5 promotes cardiac hypertrophy through translational control of SUZ12
Organisms Mus musculus; Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary The methyltransferase-like5 (METTL5), which catalyzes m6A in 18S rRNA at position A1832, has been shown to regulate the efficient of mRNA translation in the differentiation of ES cell and the growth of cancer cells. It remains unknown that whether and how METTL5 regulates cardiac hypertrophy. In this study, we generated a mouse model (METTL5-cKO) with cardiac-specific abolishment of METTL5 in vivo. Loss function of METTL5 promotes pressure overload-induced cardiomyocyte hypertrophy and adverse remodeling. The regulatory function of METTL5 in hypertrophic growth of cardiomyocyte were further confirmed with both gain- and loss-of-function approaches in primary isolated cardiomyocytes. Mechanically, METTL5 was identified to modulate the mRNA translation of SUZ12, a core component of PRC2 complex, and further regulate the transcriptome shift during cardiac hypertrophy. Therefore, our study uncover an important translational regulator of cardiac hypertrophy.
 
Overall design heart mRNA profiles of 1-month old Ctrl and Mettl5-KO mice; heart mRNA profiles of 3-month old Ctrl and Mettl5-cKO mice; heart mRNA profiles of Ctrl and Mettl5-cKO mice 4 weeks post-TAC; mRNA profiles of AdGFP and AdMettl5 NRVMs under PE stimulation.
 
Contributor(s) Han Y, Du T, Guo S, Huang Z
Citation(s) 35295259
Submission date Oct 26, 2021
Last update date Mar 30, 2022
Contact name Tailai Du
E-mail(s) dutlai@mail2.sysu.edu.cn
Organization name Sun Yat-sen University
Department The First Affiliated Hospital
Lab Institute of Precision Medicine
Street address No.58, Zhongshan 2nd road
City Guangzhou
State/province Guangdong
ZIP/Postal code 510080
Country China
 
Platforms (2)
GPL23479 BGISEQ-500 (Mus musculus)
GPL24782 BGISEQ-500 (Rattus norvegicus)
Samples (32)
GSM5657799 Heart Ctrl1 rep1
GSM5657800 Heart Ctrl1 rep2
GSM5657801 Heart Ctrl1 rep3
Relations
BioProject PRJNA774794
SRA SRP343256

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Supplementary file Size Download File type/resource
GSE186615_1-month_old_Ctrl_and_Mettl5-KO_mice.csv.gz 782.8 Kb (ftp)(http) CSV
GSE186615_3-month_old_Ctrl_and_Mettl5-KO_mice.csv.gz 784.7 Kb (ftp)(http) CSV
GSE186615_PE_AdGFP_and_AdMettl5_NRVM.csv.gz 540.5 Kb (ftp)(http) CSV
GSE186615_TAC_Ctrl_and_Mettl5-KO_mice.csv.gz 781.9 Kb (ftp)(http) CSV
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Processed data are available on Series record

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