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| Status |
Public on Nov 02, 2021 |
| Title |
PRMT5 deficiency enforces the transcriptional and epigenetic programs of Klrg1+CD8+ terminal effector T cells [scRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Protein arginine methyltransferase 5 (PRMT5) participates in symmetric dimethylation of arginine residues of proteins and contributes to a wide range of biological processes. But how PRMT5 affects the transcriptional and epigenetic programs involved in the establishment and maintenance of T cell subsets differentiation and roles in antitumor immunity are still incompletely understood. Here, using the single cell RNA sequencing, CHIP-sequencing and bulk RNA sequencing, we found that mice T cell-specific deletion of PRMT5 had greater effects on CD8+ than CD4+ T cells development, enforcing CD8+ T cells differentiation into Klrg1+ terminal effector cells. Mechanically, T cells deficiency of PRMT5 activated Prdm1 by decreasing H4R3me2s and H3R8me2s deposition on its loci, which promote differentiation of Klrg1+CD8+ T cells. Furthermore, effector CD8+ T cells that transited into memory precursor cells were decreased in PRMT5 deficiency T cells thus caused dramatic CD8+ T cells death.
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| Overall design |
CD3+ T cells were isolated from control (WT) (n=3) and PRMT5 CKO (CRE) (n=3) mice spleens and used for single cell RNA sequencing.
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| Contributor(s) |
Zheng Y, Chen Z |
| Citation(s) |
34911774 |
| Submission date |
Oct 27, 2021 |
| Last update date |
Feb 01, 2022 |
| Contact name |
zheyi chen |
| E-mail(s) |
zheyi_chen0601@163.com, chenzheyi@sjtu.edu.cn
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| Organization name |
Shanghai Jiao Tong University School of Medicine
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| Street address |
1665 Kong Jiang Road
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| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE186863 |
PRMT5 deficiency enforces the transcriptional and epigenetic programs of Klrg1+CD8+ terminal effector T cells |
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| Relations |
| BioProject |
PRJNA775082 |
| SRA |
SRP343440 |
