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| Status |
Public on May 01, 2024 |
| Title |
High-throughput mapping of 2'-O-methylation in Trypanosoma brucei poly(A) RNAs using RibOxi-seq |
| Organism |
Trypanosoma brucei |
| Experiment type |
Other
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| Summary |
In this study we used RibOxi-seq to identify 2’-O-methylation (Nm) present on mRNAs in single nucleotide resolution. Our analysis identified hundreds of Nm sites in mRNAs, predominantly in CDS and 3’ UTR.
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| Overall design |
Trypanosoma brucei procyclic form was used to extract total RNA. The RNA was then polyA enriched before going through RibOxi-seq for 2'-O-methylation profiling
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| Contributor(s) |
Zhu Y, Rajan KS, Michaeli S, Holley C |
| Citation missing |
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| |
| Submission date |
Nov 01, 2021 |
| Last update date |
May 01, 2024 |
| Contact name |
Christopher L Holley |
| Organization name |
Duke University
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| Street address |
2301 Erwin Rd
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27705 |
| Country |
USA |
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| Platforms (1) |
| GPL20572 |
Illumina NextSeq 500 (Trypanosoma brucei) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA776821 |
| SRA |
SRP344023 |
