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| Status |
Public on Aug 08, 2023 |
| Title |
A feedback loop between heterochromatin and the nucleopore complex controls germ-cell to oocyte transition during Drosophila oogenesis [Cut&Run] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Germ cells differentiate into oocytes that become totipotent upon fertilization. How the highly specialized oocyte acquires this distinct cell fate is poorly understood. During Drosophila oogenesis, H3K9me3 histone methyltransferase SETDB1 translocates from the cytoplasm to the nucleus of germ cells concurrent with oocyte specification. Here, we discovered that nuclear SETDB1 is required to silence a cohort of differentiation-promoting genes by mediating their heterochromatinization. Intriguingly, SETDB1 is also required for the upregulation of 18 of the ~30 nucleoporins (Nups) that comprise the nucleopore complex (NPC). NPCs in turn anchor SETDB1-dependent heterochromatin at the nuclear periphery to maintain H3K9me3 and gene silencing in the egg chambers. Aberrant gene expression due to loss of SETDB1 or Nups results in loss of oocyte identity, cell death and sterility. Thus, a feedback loop between heterochromatin and NPCs promotes transcriptional reprogramming at the onset of oocyte specification that is critical to establish oocyte identity.
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| Overall design |
Sequencing of duplicate samples after performing CUT&RUN of ovaries from WT, germline SETDB1 KD and Nup154 KD for H3K9me3
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| Contributor(s) |
Sarkar K, Kotb N, Lemus A, Martin ET, McCarthy A, Camacho J, Iqbal A, Valm AM, Sammons M, Rangan P |
| Citation missing |
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| |
| Submission date |
Nov 02, 2021 |
| Last update date |
Aug 09, 2023 |
| Contact name |
Prashanth Rangan |
| E-mail(s) |
ranganlabualbany@gmail.com
|
| Phone |
5184423485
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| Organization name |
RNA Institute
|
| Department |
Biological Sciences
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| Lab |
Rangan Lab
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| Street address |
1400 Washington
|
| City |
Albany |
| State/province |
NY |
| ZIP/Postal code |
12222 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE186982 |
A feedback loop between heterochromatin and the nucleopore complex controls germ-cell to oocyte transition during Drosophila oogenesis |
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| Relations |
| BioProject |
PRJNA777060 |
| SRA |
SRP344198 |
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