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| Status |
Public on Apr 13, 2022 |
| Title |
SETDB1 interactions with PELP1 contributes to breast cancer endocrine therapy resistance via Akt activation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, we examined the mechanisms by which SETDB1 regulate the cell viability of estrogen receptor (ER) positive breast cancer cells. MCF7 cells were stably transfected with non-targeted shRNA or SETDB1 shRNA via lentiviral transduction. Total RNA was isolated and utilized for RNA-seq analysis. Our results demonstrated that SETDB1 regulates the expression of subsets of genes involved in ER-and Akt-signaling.
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| Overall design |
Total RNA from MCF7 control-shRNA and SETDB1-shRNA cells were isolated using RNeasy mini kit (Qiagen). For whole-genome transcriptome profiling, 2 libraries (3 replicates each for knockdown and control) were generated from total RNA from non-targeted shRNA and SETDB1-shRNA transfected MCF7 cells, using a TruSeq Stranded mRNA Library Preparation kit according to the manufacturer’s protocol (Illumina Inc.). Samples were sequenced on the Illumina HiSeq 3000 platform (Illumina Inc.) using the 50 base-pair single-read (50SR) sequencing module.
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| Contributor(s) |
Vadlamudi RK, Chen Y, Lai Z |
| Citation(s) |
35395812 |
| |
| Submission date |
Nov 03, 2021 |
| Last update date |
Apr 14, 2022 |
| Contact name |
Yidong Chen |
| E-mail(s) |
cheny8@uthscsa.edu
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| Phone |
2105629163
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| Organization name |
UT Health Science Center at San Antonio
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| Department |
Population Health Sciences
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| Street address |
8403 Floyd Curl Drive, MSC 7784
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| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA777551 |
| SRA |
SRP344480 |
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