|
Status |
Public on Oct 18, 2010 |
Title |
MicroRNA expression profiling in adult mouse liver following benzo(a)pyrene (BaP) treatment [Exiqon miRNA array] |
Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae |
Sample organism |
Mus musculus |
Experiment type |
Non-coding RNA profiling by array
|
Summary |
Lack of change in microRNA expression in adult mouse liver following treatment with benzo(a)pyrene (BaP), as detected using Exiqon miRNA arrays. Adult male mice were exposed to 150 mg/kg benzo(a)pyrene (BaP) or solvent for 3 days and sampled 4 hours after the last dose. MicroRNA expression levels in adult mouse liver were measured using Exiqon miRNA arrays. Our results indicate a distinct lack of effect of BaP of miRNA expression, despite widespread changes in mRNA levels (measured using Agilent arrays). Lack of miRNA changes was confirmed with Agilent miRNA arrays.
Keywords: Toxicology, miRNA
|
|
|
Overall design |
Adult male B6C3F1 mice were exposed to a daily dose of corn oil (vehicle control group) or 150 mg/kg BaP (treatment group) for 3 d (n=6 per group). Mice were sacrificed at 4 h or 24 h following the last dose and liver lobes were extracted and flash frozen. Exiqon miRNA arrays were used to examine changes in miRNA transcript levels in random liver lobe sections.
|
|
|
Contributor(s) |
Yauk CL, Jackson KA |
Citation(s) |
20188852 |
|
Submission date |
Oct 28, 2009 |
Last update date |
May 10, 2016 |
Contact name |
Andrew Williams |
Organization name |
Health Canada
|
Street address |
50 Columbine
|
City |
Ottawa |
State/province |
ON |
ZIP/Postal code |
K1A OK9 |
Country |
Canada |
|
|
Platforms (1) |
GPL7723 |
miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno |
|
Samples (12)
|
|
This SubSeries is part of SuperSeries: |
GSE18841 |
Adult mouse liver following benzo(a)pyrene (BaP) treatment: miRNA and mRNa profiling |
|
Relations |
BioProject |
PRJNA123859 |