|
| Status |
Public on Aug 10, 2022 |
| Title |
A Notch-dependent transcriptional mechanism controls expression of temporal patterning factors in Drosophila medulla |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In this work, we show that the expression of one temporal transcription factor(TTF), Sloppy-paired (Slp), in the Drosophila medulla TTF cascade, is regulated directly by two other TTFs and the Notch signaling pathway. We show that the previous TTF, Eyeless, another TTF Scro and Su(H)/NICD regulate the transcription of Slp through binding to two cis-regulatory elements in the slp locus. Slp expression is delayed when Notch signaling is lost or when cell cycle progression is blocked, which also cause loss of Notch signaling. Furthermore, we show that supplying Notch signaling can rescue the delayed Slp expression in cell-cycle arrested neuroblasts. Thus our work demonstrates that Notch signaling cooperates with TTFs to promote the progression of the TTF transcriptional cascade.
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| |
|
| Overall design |
Dam-ID sequencing to profile the binding sites of Su(H) and Ey
|
| |
|
| Contributor(s) |
Li X, Ray A |
| Citation(s) |
36040415 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 EY026965 |
Temporal patterning of neural progenitors to generate neural diversity |
UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN |
Xin Li |
|
| |
| Submission date |
Nov 11, 2021 |
| Last update date |
Sep 15, 2022 |
| Contact name |
Xin Li |
| E-mail(s) |
lixin@illinois.edu
|
| Phone |
2172443784
|
| Organization name |
University of Illinois Urbana-Champaign
|
| Department |
CDB
|
| Lab |
Li
|
| Street address |
601 S. Goodwin Avenue, B107 CLSL
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA779739 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE188643_Ey_1-vs-Dam.gatc-FDR0.01.peaks.gff.gz |
20.2 Kb |
(ftp)(http) |
GFF |
| GSE188643_Ey_1-vs-Dam.gatc.bedgraph.gz |
4.7 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE188643_Ey_2-vs-Dam.gatc-FDR0.01.peaks.gff.gz |
20.8 Kb |
(ftp)(http) |
GFF |
| GSE188643_Ey_2-vs-Dam.gatc.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE188643_Ey_and_SuH_overlap_allpeaks.gff.gz |
43.3 Kb |
(ftp)(http) |
GFF |
| GSE188643_Ey_idr.gff.gz |
10.3 Kb |
(ftp)(http) |
GFF |
| GSE188643_Ey_overlap_all_replicate_peaks.gff.gz |
31.2 Kb |
(ftp)(http) |
GFF |
| GSE188643_SuH_1-vs-Dam.gatc-FDR0.01.peaks.gff.gz |
19.9 Kb |
(ftp)(http) |
GFF |
| GSE188643_SuH_1-vs-Dam.gatc.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE188643_SuH_2-vs-Dam.gatc-FDR0.01.peaks.gff.gz |
20.2 Kb |
(ftp)(http) |
GFF |
| GSE188643_SuH_2-vs-Dam.gatc.bedgraph.gz |
4.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE188643_SuH_idr.gff.gz |
11.0 Kb |
(ftp)(http) |
GFF |
| GSE188643_SuH_overlap_all_replicate_peaks.gff.gz |
31.4 Kb |
(ftp)(http) |
GFF |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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