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| Status |
Public on Nov 01, 2022 |
| Title |
Serine Palmitoyltransferase Controls Stemness of Intestinal Progenitors [single cell RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cancers of the gastrointestinal tract including esophageal adenocarcinomas, colorectal cancers, and cancers of the gastric cardia are common comorbidities of obesity. Excessive delivery of macronutrients to the cells lining the gut can increase one’s risk for these cancer by inducing imbalances in the rate of intestinal stem cell proliferation vs. differentiation, which can produce polyps and other aberrant growths. We demonstrate that serine palmitoyltransferase (SPT), which diverts dietary fatty and amino acids into the sphingolipid biosynthesis pathway, is a critical modulator of intestinal stem cell homeostasis. SPT and other enzymes in the biosynthetic pathway are upregulated in human colon tumors. These enzymes produce sphingolipids that serve as pro-stemness signals that stimulate peroxisome-proliferator activated receptor alpha (PPARa)-mediated induction of fatty acid binding protein-1. This increases fatty acid uptake and oxidation and enhances the stemness program. Serine palmitoyltransferase thus serves as a critical link between dietary macronutrients, epithelial regeneration, and cancer risk.
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| Overall design |
Examination of 2 different treatments in intestinal organoids.
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| Contributor(s) |
Li Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 12, 2021 |
| Last update date |
Nov 03, 2022 |
| Contact name |
Ying Li |
| E-mail(s) |
li.ying@utah.edu
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| Phone |
8015850860
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| Organization name |
University of Utah
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| Department |
NUIP
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| Lab |
Scott Summers
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| Street address |
15 North 2030 East, Eccles Institute of Human Genetics
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| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE188676 |
Serine Palmitoyltransferase Controls Stemness of Intestinal Progenitors. |
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| Relations |
| BioProject |
PRJNA779928 |
| SRA |
SRP345753 |
