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Series GSE188675 Query DataSets for GSE188675
Status Public on Nov 01, 2022
Title Serine Palmitoyltransferase Controls Stemness of Intestinal Progenitors [single cell RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Cancers of the gastrointestinal tract including esophageal adenocarcinomas, colorectal cancers, and cancers of the gastric cardia are common comorbidities of obesity. Excessive delivery of macronutrients to the cells lining the gut can increase one’s risk for these cancer by inducing imbalances in the rate of intestinal stem cell proliferation vs. differentiation, which can produce polyps and other aberrant growths. We demonstrate that serine palmitoyltransferase (SPT), which diverts dietary fatty and amino acids into the sphingolipid biosynthesis pathway, is a critical modulator of intestinal stem cell homeostasis. SPT and other enzymes in the biosynthetic pathway are upregulated in human colon tumors. These enzymes produce sphingolipids that serve as pro-stemness signals that stimulate peroxisome-proliferator activated receptor alpha (PPARa)-mediated induction of fatty acid binding protein-1. This increases fatty acid uptake and oxidation and enhances the stemness program. Serine palmitoyltransferase thus serves as a critical link between dietary macronutrients, epithelial regeneration, and cancer risk.
Overall design Examination of 2 different treatments in intestinal organoids.
Contributor(s) Li Y
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Submission date Nov 12, 2021
Last update date Nov 03, 2022
Contact name Ying Li
Phone 8015850860
Organization name University of Utah
Department NUIP
Lab Scott Summers
Street address 15 North 2030 East, Eccles Institute of Human Genetics
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM5687955 18761X1 Sptlcfl/fl
GSM5687956 18761X2 Sptlc2δIEC
This SubSeries is part of SuperSeries:
GSE188676 Serine Palmitoyltransferase Controls Stemness of Intestinal Progenitors.
BioProject PRJNA779928
SRA SRP345753

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Supplementary file Size Download File type/resource
GSE188675_RAW.tar 162.9 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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