|
| Status |
Public on Dec 31, 2021 |
| Title |
Integrative analysis of lncRNAs, mRNAs and miRNAs indicates the neurodevelopmental dysfunction in the hippocampus of gut microbiota-dysbiosis mice [miRNA] |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Major depressive disorder is caused by gene-environment interactions and the gut microbiota plays a pivotal role in the development of depression. However, the mechanisms by which the gut microbiota modulates depression remain elusive. Herein, we detected the differentially expressed hippocampal long non-coding RNAs (lncRNAs), messenger RNAs (mRNAs) and microRNAs (miRNAs) between mice inoculated with gut microbiota from major depressive disorder patients or healthy controls, to identify the effects of gut microbiota-dysbiosis on gene regulation patterns at the transcriptome level. We also performed functional analysis to explore the microbial-regulated pathological mechanisms of depression. Two hundred mRNAs, 358 lncRNAs and 4 miRNAs were differentially expressed between the two groups. Functional analysis of these differentially expressed mRNAs indicated dysregulated inflammatory response to be the primary pathological change. Intersecting the differentially expressed mRNAs with targets of differentially expressed miRNAs identified 47 intersected mRNAs, which were mainly related to neurodevelopment. Additionally, we constructed a microbial-regulated lncRNA-miRNA-mRNA network based on RNA-RNA interactions. According to the competitive endogenous RNA hypothesis, two neurodevelopmental ceRNA sub-networks implicating in depression were identified. This study provides new understanding of the pathogenesis of depression induced by gut microbiota-dysbiosis and may act as a theoretical basis for the development of gut microbiota-based antidepressants.
|
| |
|
| Overall design |
we performed lncRNA, mRNA and miRNA expression profiling in the hippocampus of mice that had been inoculated with “depression microbiota” (microbiota from fecal samples of MDD patients) or “healthy microbiota” (microbiota from fecal samples of healthy controls)
|
| |
|
| Contributor(s) |
Liu L, Wang H |
| Citation(s) |
35087377 |
| |
| Submission date |
Nov 18, 2021 |
| Last update date |
Feb 02, 2022 |
| Contact name |
Anna Liu |
| E-mail(s) |
254445357@qq.com
|
| Organization name |
Chongqing Medical University
|
| Street address |
1 Youyi Road, Yuzhong Dist.
|
| City |
Chongqing |
| ZIP/Postal code |
400016 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL18058 |
Exiqon miRCURY LNA microRNA array, 7th generation [miRBase v18, condensed Probe_ID version] |
|
| Samples (6)
|
| GSM5695537 |
Mice inoculated with gut microbiota from major depressive disorder patients, rep 1 |
| GSM5695538 |
Mice inoculated with gut microbiota from major depressive disorder patients, rep 2 |
| GSM5695539 |
Mice inoculated with gut microbiota from major depressive disorder patients, rep 3 |
| GSM5695540 |
Mice inoculated with gut microbiota from healthy controls, rep 1 |
| GSM5695541 |
Mice inoculated with gut microbiota from healthy controls, rep 2 |
| GSM5695542 |
Mice inoculated with gut microbiota from healthy controls, rep 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE189234 |
Integrative analysis of lncRNAs, mRNAs and miRNAs indicates the neurodevelopmental dysfunction in the hippocampus of gut microbiota-dysbiosis mice |
|
| Relations |
| BioProject |
PRJNA782104 |
Less...
More...