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| Status |
Public on Nov 02, 2022 |
| Title |
Stromal inflammation is a targetable driver of blood aging [droplet-based scRNAseq - Batch 2] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the role of IL1 signaling in hematopoietic aging, we performed droplet-based scRNAseq (10X Genomics) of hematopoietic stem and progenitor cells (LK/LSK) and unfractionated endosteal and central marrow stromal cells (Ter119-/CD45-) from young wild type and young and old wild type and IL1R1-/- C57BL/6 mice.
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| Overall design |
Hematopoietic stem and progenitor cells (lineage negative, cKit positive (LK) and lineage negative, cKit positive, Sca-1 positive (LSK)) and endosteal and central marrow stromal cells from the bone marrow of young (10 weeks) and old (24 months) wild-type C57BL/6 mice were isolated by flow cytometry and subjected to droplet-based scRNAseq (10X Genomics).
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| Contributor(s) |
Mitchell CA, Olson OC, Calero-Nieto FJ, Gottgens B, Passegue E |
| Citation(s) |
36650381 |
| |
| Submission date |
Nov 19, 2021 |
| Last update date |
Feb 02, 2023 |
| Contact name |
Emmanuelle Passegue |
| E-mail(s) |
ep2828@cumc.columbia.edu
|
| Organization name |
Columbia University Irving Medical Center
|
| Department |
Columbia Stem Cell Initiative
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| Street address |
650 W 168th St
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE169162 |
Stromal niche inflammation mediated by IL-1 signaling is a targetable driver of hematopoietic aging |
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| Relations |
| BioProject |
PRJNA781994 |
| SRA |
SRP346956 |
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