The study of nascent RNA transcripts in asynchronous cells and cells released from mitosis at the indicated timepoints between Smarce1- MD (R42A) and Smarce1- MD mESCs.
Expression profiling by high throughput sequencing
Summary
SMARCE1 binding on mitotic chromatin is required for the timely reinitiation of expression of SMARCE1-bookmarked genes and maintaining identity memory in cell division.
Overall design
To examine the effect of mitosis-specific degradation of SMARCE1 on transcription reactivation following exit from mitosis, we used 5- ethynyluridine (EU) to pulse-label nascent transcripts in Smarce1-MD (R42A) (clone#A04, #A10) and Smarce1-MD (clone#MD09, #MD30) mESCs. Pulse-labeled transcripts were analyzed at 0, 45, 90, 180, and 240 min after nocodazole washout as well as in asynchronous cells.
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