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Series GSE189589 Query DataSets for GSE189589
Status Public on Aug 23, 2022
Title Genome wide mapping of RBP95-HTP binding sites in yeast by CRAC
Organism Saccharomyces cerevisiae
Experiment type Other
Summary We report the application of the Cross-Linking and cDNA (CRAC) technique to identify binding sites of RBP95, ribosome assembly factors, on RNAs in vivo. Once sequenced, Rnas associated have been aligned to the yeast genomes and enrichment of specific RNAs has been studied in more details. Specifically apparition of Mutation/deletion in the reads are of interesdt since they mostly corresponds to prceise site of cross-link between bait protein and target RNA. Here RBP95-HTP is mostly bound to 25S rRNA at one partiuclar site and to several snoRNAs, including SNR37, SNR30, SNR190, SNR86 and SNR128.
 
Overall design We compare sequences enrichement of RNAs associated to Nap1_HTP or to a mock (un-tagged) control after protein/RNA cross-link in vivo.
 
Contributor(s) Lebaron S, Henras AK
Citation(s)
  • Bhutada P, Favre S, Jaafar M, Hafner J et al. Rbp95 binds to 25S rRNA helix H95 and cooperates with the Npa1 complex during early pre-60S particle maturation. Nucleic Acids Res 2022 Sep 23;50(17):10053-10077. PMID: 36018804
Submission date Nov 26, 2021
Last update date Oct 21, 2022
Contact name Simon Lebaron
E-mail(s) simonleba@gmail.com
Organization name Université Paris Descartes
Department LCRB-UMR8015
Lab Leulliot
Street address 4 avenue de l'observatoire
City PARIS
ZIP/Postal code 75270
Country France
 
Platforms (1)
GPL17143 Illumina MiSeq (Saccharomyces cerevisiae)
Samples (2)
GSM5704226 BY4741_control_1
GSM5704227 RBP95-HTP
Relations
BioProject PRJNA783799
SRA SRP348004

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE189589_RAW.tar 60.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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