NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE189744 Query DataSets for GSE189744
Status Public on Apr 30, 2023
Title Gene expression analysis of mouse brain.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Inbred mouse strains differ in their innate anxiety levels. We used RNA-sequencing to identify gene expression differences in hippocampus and frontal cortex of six mouse strains.
 
Overall design Mice were singly housed for one week before dissections and killed by cervical dislocation between 8 a.m. and 11 a.m. Dissections were performed on a Petri dish filled with ice. FCx included 2 mm of the anterior part of the cortex. The Hpc was dissected whole. Samples were directly frozen in liquid nitrogen and stored in -80°C. We extracted total RNA with TriReagent according to the manufacturer’s instructions and verified its quality by the Agilent Bioanalyzer Small RNA Analysis and RNA 6000 Nano Kits. Ribo-depleted RNA was sequenced with Illumina High-Seq 2000.
 
Contributor(s) Hovatta I
Citation(s)
  • Gigliotta A, Trontti K, Väänänen J, Hovatta I. Gene expression profiling reveals a role of immune system and inflammation in innate and stress-induced anxiety-like behavior. Front Genet 2023;14:1173376. PMID: 37260777
Submission date Nov 28, 2021
Last update date Jul 31, 2023
Contact name Iiris Hovatta
E-mail(s) iiris.hovatta@helsinki.fi
Organization name University of Helsinki
Department Department of Psychology and Logopedics
Lab Neurogenomics
Street address Haartmaninkatu 3
City Helsinki
ZIP/Postal code 00014
Country Finland
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (36)
GSM5706640 129S1_C5
GSM5706641 129S1_C6
GSM5706642 129S1_C7
Relations
BioProject PRJNA784307
SRA SRP348281

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE189744_FCx_counts_logCPM.txt.gz 2.0 Mb (ftp)(http) TXT
GSE189744_Hpc_counts_logCPM.txt.gz 2.0 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.
HHS Vulnerability Disclosure