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Series GSE189753 Query DataSets for GSE189753
Status Public on Sep 26, 2022
Title Single cell transcriptomic of murine pancreatic tumor model by treatment of PD-L1-directed PlGF/VEGF blockade
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We developed a multi-paratopic VEGF decoy receptor (Ate-Grab) by fusing the scFv of atezolizumab to VEGF-Grab to target PD-L1 expressing cells in tumor microenvironment. We compared the single-cell transcriptomes of gemcitabine-treated and Ate-Grab with Gemcitabine-treated Pan02 tumors. We confirmed the subtype of cancer-associated fibroblasts(CAFs) that modulates the collagen inside the tumor microenvrionment. In the Ate-Grab+Gemcitabine group, it was confirmed that new CAFs were regulated.
 
Overall design Orthotopic murine pancreatic cancer models using Pan02 cells were generated. They were treated by Gemcitabine alone or Ate-Grab with Gemcitabine for 5 times every 3 days. Five gemcitabine-treated tumors and four tumors treated with Ate-Grab + gemcitabine were pooled and subjected to single-cell RNA sequencing (scRNA-seq) analysis
 
Contributor(s) Kim DK, Jeong J, Lee DS, Hyeon DY, Jeon S, Hwang D, Kim HM, Jung K
Citation(s) 36272973
Submission date Nov 29, 2021
Last update date Nov 29, 2022
Contact name Duk Ki Kim
Organization name Seoul National University of Hospital
Street address Daehakro 103
City Seoul
ZIP/Postal code 82
Country South Korea
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (3)
GSM5706741 ATG+GEM
GSM5706742 GEM
GSM6448648 Control
Relations
BioProject PRJNA784406
SRA SRP348342

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE189753_RAW.tar 350.5 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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