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| Status |
Public on Dec 04, 2021 |
| Title |
MicroRNAs Mediate Insecticide Tolerance in Spodoptera frugiperda |
| Organism |
Spodoptera frugiperda |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Spodoptera frugiperda is the world’s major agricultural pests, and has the distinctive features of high fecundity, strong migratory capacity and high resistance to most insecticides. At present, the control of S. frugiperda in China relies mainly on the spraying of chemical insecticides. MicroRNAs (miRNAs) are a class of small, single-stranded, non-coding RNAs, and play crucial regulatory roles in various physiological processes, including the insecticide resistance in insects. However, little is known about the regulatory roles of miRNAs on the resistance of S. frugiperda to insecticides. In the present research, the miRNAs that were differentially expressed after cyantraniliprole, spinetoram, emamectin benzoate and tetraniliprole treatment were analyzed by RNA-Seq. A total of 504 miRNAs were systematically identified from S. frugiperda, and 24, 22, 31 and 30 miRNAs were differentially expressed after treatments of cyantraniliprole, spinetoram, emamectin benzoate and tetraniliprole. GO and KEGG enrichment analyses were used to predict the function of differentially expressed miRNAs’ target genes. Importantly, ten miRNAs were significantly differentially expressed among the treatments of three insecticides. MiR-278-5p, miR-13b-3p, miR-10485-5p and miR-10483-5p were significantly down-regulated among the treatments of three insecticides by RT-qPCR. Furthermore, overexpression of miR-278-5p, miR-13b-3p, miR-10485-5p and miR-10483-5p significantly increased the mortality of S. frugiperda to cyantraniliprole and emamectin benzoate. The mortality was significantly increased with spinetoram treatment after overexpression of miR-13b-3p, miR-10485-5p and miR-10483-5p. These results suggest that miRNAs, which are differentially expressed in response to insecticides, may play a key regulatory role in the insecticide resistance in S. frugiperda.
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| Overall design |
The miRNAs that were differentially expressed after cyantraniliprole, spinetoram, emamectin benzoate and tetraniliprole treatment were analyzed in Spodoptera frugiperda.
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| Contributor(s) |
Zhang J, Yang Y, Zhang Y |
| Citation(s) |
35126473 |
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| Submission date |
Dec 02, 2021 |
| Last update date |
Feb 08, 2022 |
| Contact name |
jianhua zhang |
| E-mail(s) |
zhangjianhua198904@126.com
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| Organization name |
Shandong Academy of Agricultural Sciences
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| Street address |
gongyebei street No. 23788
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| City |
Jinan |
| ZIP/Postal code |
250100 |
| Country |
China |
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| Platforms (1) |
| GPL25030 |
Illumina HiSeq 2500 (Spodoptera frugiperda) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA785443 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE189968_Processed_data.xlsx |
85.9 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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