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| Status |
Public on Jun 22, 2022 |
| Title |
Transcriptome analysis of nascent hematopoietic stem cells (HSCs) induced by the ectopic expression of Evi1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To examine the role of Evi1 in HSC specification, we generated Tie2-Cre::ROSA-Evi1 mice, which can induce Evi1 in endothelial cells. We observed five-fold increase of HSCs In Tie2-Cre::ROSA-Evi1 embryo.
To characterize the induced HSCs in Tie2-Cre::ROSA-Evi1 embryos, we analyzed the gene expression profile of HSCs.
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| Overall design |
HSCs were isolated from Tie2-Cre::ROSA-Evi1 and ROSA-Evi1 fetal livers, and were subjected to RNA-seq analysis.
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| Contributor(s) |
Yokomizo T, Suda T |
| Citation(s) |
36104564 |
| |
| Submission date |
Dec 02, 2021 |
| Last update date |
Sep 23, 2022 |
| Contact name |
Tomomasa Yokomizo |
| E-mail(s) |
tomoyokomizo@gmail.com
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| Organization name |
Kumamoto University
|
| Street address |
2-2-1 Honjo, Chuo-ku
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| City |
Kumamoto |
| ZIP/Postal code |
860-0811 |
| Country |
Japan |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA785583 |
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