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Status |
Public on Aug 29, 2023 |
Title |
Maternal Circulating miRNAs Contribute to Negative Pregnancy Outcomes by Altering Placental Transcriptome and Fetal Vascular Dynamics |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We performed RNA sequencing on the fetal portion of murine placentas isolated at gestational day (GD) 18, 8 days after dams are exposed to a single intravascular administration of either pooled murine-conserved HEamiRNAs (50 μg of pooled equimolar quantities of mmu-miR-222-5p, mmu-miR-187-5p, mmu-mir-299a, mmu-miR-491-3p, miR-760-3p, mmu-miR-671-3p, mmu-miR-449a-5p and mmu-miR-204-5p mimics) or control scrambled miRNAs.
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Overall design |
Pregnant C57Bl6/J mice served as controls or were exposed to HEamiRNAs by intravascular administration on gestational day (GD) 10. On GD18, the fetal portion of murine placentas, excluding the decidual portion (maternal), was micro-dissected. A total of 19 samples (10 male and 9 female samples), originating from 11 pregnant dams, 6 HEamiRNAs -treated and 5 control.
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Contributor(s) |
Pinson MR, Tseng AM, Adams AA, Lehman TE, Chung K, Gutierrez J, Larin KV, Chambers C, Miranda RC |
Citation(s) |
37930978 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
R01 AA024659 |
Prenatal microRNA neuro-therapeutics for fetal alcohol exposure |
The Texas A&M University System Health Science Center |
Rajesh C Miranda |
R01 HD086765 |
Optical Coherence Tomography to Study Effect of Poly - Drug Exposure on Fetal Brain Development |
University of Houston |
Rajesh C Miranda |
F30 AA027698 |
Gag-like Proteins in exosome-mediated Neural Development and Fetal Alcohol Spectrum Disorders |
The Texas A&M University System Health Science Center |
Marisa Pinson |
F31 AA026505 |
Maternal circulating miRNA function in Fetal Alcohol Spectrum Disorders |
The Texas A&M University System Health Science Center |
Alexander Mike Tseng |
U01 AA014835 |
Early Predictors of FASD in Ukraine |
THE REGENTS OF THE UNIV. OF CALIF., UNIV. OF CALIF., SAN DIEGO |
CHRISTINA CHAMBERS |
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Submission date |
Dec 02, 2021 |
Last update date |
Dec 03, 2023 |
Contact name |
Rajesh Miranda |
E-mail(s) |
rmiranda@tamu.edu
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Organization name |
Texas A&M University
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Street address |
8447 Riverside Parkway
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City |
Bryan |
ZIP/Postal code |
77807 |
Country |
USA |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (19)
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Relations |
BioProject |
PRJNA785594 |
SRA |
SRP348931 |
Supplementary file |
Size |
Download |
File type/resource |
GSE190017_DESeq2_normalized_counts_TV_ANOVA_Transcript.csv.gz |
1.9 Mb |
(ftp)(http) |
CSV |
GSE190017_DESeq2_result_file_on_TV_ANOVA_Transcript.csv.gz |
741.4 Kb |
(ftp)(http) |
CSV |
GSE190017_RAW.tar |
2.0 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
Processed data are available on Series record |
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