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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 22, 2010 |
| Title |
Genome-wide mapping of Nr5a2 in mouse embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Nr5a2 (also known as liver receptor homolog-1, Lrh-1) has been shown to bind both the proximal enhancer and proximal promoter regions of Pou5f1 and regulate Pou5f1 in the epiblast stage of mouse embryonic development (Gu et al., 2005). Nr5a2-null embryos display a loss of Oct4 expression in the epiblasts (Gu et al., 2005) and die between E6.5 and E7.5 (Gu et al., 2005; Pare et al., 2004). To identify the targets of Nr5a2, we generated a stable ES cell-line that expresses HA-tagged Nr5a2. Anti-HA antibody was used to immunoprecipitate HA-Nr5a2 for ChIP-seq analysis.
Keywords: Transcription factor binding sites
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| Overall design |
To identify the binding sites of Nr5a2, we generated a stable ES cell-line that expresses HA-tagged Nr5a2. Anti-HA antibody was used to immunoprecipitate HA-Nr5a2.
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| Contributor(s) |
Heng D, Ng HH |
| Citation(s) |
20096661 |
| Submission date |
Nov 13, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Mikael Huss |
| E-mail(s) |
hussem@gis.a-star.edu.sg
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| Phone |
+6564788042
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| Organization name |
Genome Institute of Singapore
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| Street address |
60 Biopolis Street
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| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (2) |
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| Relations |
| SRA |
SRP001796 |
| BioProject |
PRJNA120611 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19019_Nr5a2_peaks_1e-9.bed.gz |
29.5 Kb |
(ftp)(http) |
BED |
| GSE19019_RAW.tar |
1.1 Gb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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