|
| Status |
Public on Dec 10, 2021 |
| Title |
Translocated Legionella pneumophila small RNAs mimic eukaryotic miRNAs to dampen the host immune response [THP-1] |
| Organisms |
Legionella pneumophila; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Extracellular vesicles (EVs) produced by bacteria, archaea and eukaryotic cells, are increasingly recognized as important mediators of intercellular communication via transfer of a wide variety of molecular cargoes. To characterize the effect of Lp-EVs on THP-1 cells, in particular the immune response, three independent RNAseq libraries were constructed from from THP-1 cells non-infected, incubated with purified wt-EVs or with purified EV from delta-rsmY Legionella bacteria. The RNA deep was purified after 3h of incubation and sequenced using an Illumina platform.
|
| |
|
| Overall design |
For each RNAseq experiment, the transcription of THP-1 cells incubated with extracellular vesicles deriving from wt and delta-rsmY Legionella bacteria or form the non-infected control experiment was compared. In total n=3 independent experiments were performed
|
| |
|
| Contributor(s) |
Sahr T, Escoll Guerrero P, Rusniok C, Buchrieser C |
| Citation(s) |
35140216 |
| |
| Submission date |
Dec 07, 2021 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Christophe Rusniok |
| E-mail(s) |
rusniok@pasteur.fr
|
| Organization name |
Institut Pasteur
|
| Department |
Genomes et Genetique
|
| Lab |
Unite de Biologie des Bacteries Intracelulaires
|
| Street address |
25, 28 rue du Docteur Roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
|
| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL31034 |
Illumina HiSeq 2500 (Homo sapiens; Legionella pneumophila) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA786901 |
| SRA |
SRP349653 |
Less...
More...