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Status |
Public on Aug 20, 2010 |
Title |
Germfree C57BL/6J mice are resistant to high fat diet-induced insulin resistance and have altered cholesterol metabolism |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Germfree (GF) mice have been used as a model to study the contribution of the intestinal microbiota to metabolic energy balance of the host. Despite a wealth of knowledge accumulated since the 1940’s, the response of GF mice to a high fat diet is largely unknown. In the present study, we compared the metabolic consequences of a high fat (HF) diet on GF and conventional (Conv) C57BL/6J mice. As expected, Conv mice developed obesity and glucose intolerance with a HF diet. In contrast, GF mice remained lean and resisted the HF diet-induced insulin resistance. The anti-obesity phenotype of GF/HF mice was accompanied by reduced caloric intake, diminished food efficiency, and excessive fecal lipid excretion contributed to the reduced food efficiency. In addition, HF diet-induced hypercholesterolemia was ameliorated, which was partially due to an increase in fecal cholesterol excretion. However, hepatic cholesterols were increased in GF/HF mice. Elevated nuclear SREBP2 proteins and the up-regulation of cholesterol biosynthesis genes support the increased liver cholesterol biosynthesis in GF/HF mice. The resistance to HF diet-induced metabolic abnormalities in GF mice was also associated with a reduced immune response, indicated by low plasma pro-inflammatory and anti-inflammatory markers. These data suggest that the gut microbiota of Conv mice contributes to HF diet-induced obesity, insulin resistance, dyslipidemia and hepatic steatosis in mice. Thus, results of the present study describe the metabolic responses of GF mice to a HF diet and further our understandings of the relationship between the gut microbiota and the host.
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Overall design |
Germfree and conventional C57BL/6J mice were fed with a high fat diet for 11 weeks. Then, all mice were sacrified under 10-h food deprevation, and liver samples of germfree (n=14) and conventional (n=16) were examined.
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Contributor(s) |
Chou CJ, Raymond F, Mansourian R |
Citation(s) |
20724524 |
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Submission date |
Nov 16, 2009 |
Last update date |
Jan 18, 2013 |
Contact name |
Frederic Raymond |
E-mail(s) |
frederic.raymond@rd.nestle.com
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Organization name |
Nestle Institute of Health Sciences
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Department |
Functional Genomics
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Street address |
Campus EPFL - Quartier de l'Innovation
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City |
Lausanne |
ZIP/Postal code |
1015 |
Country |
Switzerland |
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Platforms (1) |
GPL6103 |
Illumina mouseRef-8 v1.1 expression beadchip |
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Samples (30)
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Relations |
BioProject |
PRJNA120645 |