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Series GSE19038 Query DataSets for GSE19038
Status Public on Aug 20, 2010
Title Germfree C57BL/6J mice are resistant to high fat diet-induced insulin resistance and have altered cholesterol metabolism
Organism Mus musculus
Experiment type Expression profiling by array
Summary Germfree (GF) mice have been used as a model to study the contribution of the intestinal microbiota to metabolic energy balance of the host. Despite a wealth of knowledge accumulated since the 1940’s, the response of GF mice to a high fat diet is largely unknown. In the present study, we compared the metabolic consequences of a high fat (HF) diet on GF and conventional (Conv) C57BL/6J mice. As expected, Conv mice developed obesity and glucose intolerance with a HF diet. In contrast, GF mice remained lean and resisted the HF diet-induced insulin resistance. The anti-obesity phenotype of GF/HF mice was accompanied by reduced caloric intake, diminished food efficiency, and excessive fecal lipid excretion contributed to the reduced food efficiency. In addition, HF diet-induced hypercholesterolemia was ameliorated, which was partially due to an increase in fecal cholesterol excretion. However, hepatic cholesterols were increased in GF/HF mice. Elevated nuclear SREBP2 proteins and the up-regulation of cholesterol biosynthesis genes support the increased liver cholesterol biosynthesis in GF/HF mice. The resistance to HF diet-induced metabolic abnormalities in GF mice was also associated with a reduced immune response, indicated by low plasma pro-inflammatory and anti-inflammatory markers. These data suggest that the gut microbiota of Conv mice contributes to HF diet-induced obesity, insulin resistance, dyslipidemia and hepatic steatosis in mice. Thus, results of the present study describe the metabolic responses of GF mice to a HF diet and further our understandings of the relationship between the gut microbiota and the host.
 
Overall design Germfree and conventional C57BL/6J mice were fed with a high fat diet for 11 weeks. Then, all mice were sacrified under 10-h food deprevation, and liver samples of germfree (n=14) and conventional (n=16) were examined.
 
Contributor(s) Chou CJ, Raymond F, Mansourian R
Citation(s) 20724524
Submission date Nov 16, 2009
Last update date Jan 18, 2013
Contact name Frederic Raymond
E-mail(s) frederic.raymond@rd.nestle.com
Organization name Nestle Institute of Health Sciences
Department Functional Genomics
Street address Campus EPFL - Quartier de l'Innovation
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platforms (1)
GPL6103 Illumina mouseRef-8 v1.1 expression beadchip
Samples (30)
GSM471176 Liver_Conventional_HFdiet_01
GSM471177 Liver_Conventional_HFdiet_02
GSM471178 Liver_Conventional_HFdiet_03
Relations
BioProject PRJNA120645

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19038_RAW.tar 3.4 Mb (http)(custom) TAR
GSE19038_non-normalized.txt.gz 9.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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